Summary -Peripheral blood lymphocytes from 4 cattle donors were grown for 2 cell cycles in the presence of 0.1, 0.25, 0.5, 1.0, 2.5 and 5.0 wg/ml of 5-bromodeoxyuridine in order to detect the spontaneous level of sister chromatid exchanges and the dose-response relationships at low bromodeoxyuridine levels. Sister chromatid exchanges were counted on 50 s cycle metaphase spreads, randomly scored, for each donor at each dose. The mean rate of sister chromatid exchanges/cell at 0.1 !Lg/ml of BUdR was 2.48 f 1.75, which can be considered as spontaneous. The dose-response curve increased more rapidly from 0.1 to 0.5 !g/ml of bromodeoxyuridine and less rapidly at higher concentrations. The frequency distribution of sister chromatid exchanges/cell and that of chromosomes showing various numbers of exchanges followed the Poisson probability function only within the range of 0.1 to 1.0 wg/ml of bromodeoxyuridine.
Summary -Peripheral blood lymphocytes from 4 cattle donors were grown for 2 cell cycles in the presence of 0.1, 0.25, 0.5, 1.0, 2.5 and 5.0 wg/ml of 5-bromodeoxyuridine in order to detect the spontaneous level of sister chromatid exchanges and the dose-response relationships at low bromodeoxyuridine levels. Sister chromatid exchanges were counted on 50 s cycle metaphase spreads, randomly scored, for each donor at each dose. The mean rate of sister chromatid exchanges/cell at 0.1 !Lg/ml of BUdR was 2.48 f 1.75, which can be considered as spontaneous. The dose-response curve increased more rapidly from 0.1 to 0.5 !g/ml of bromodeoxyuridine and less rapidly at higher concentrations. The frequency distribution of sister chromatid exchanges/cell and that of chromosomes showing various numbers of exchanges followed the Poisson probability function only within the range of 0.1 to 1.0 wg/ml of bromodeoxyuridine.
A differential sister chromatid exchange (SCE)/cell response was observed between phytohemagglutinin (PHA) and pokeweed (PKW) stimulated blood lymphocytes of goat (Capra hircus L) exposed to final concentrations of 0.1, 0.25, 0.5, 1, 2.5 and 5 pg/mL of BUdR. At 0.1 !g/mL of BUdR, the two mitogens gave very similar SCE/cell responses: the SCE mean values were 3.17 ! 1.93 for PHA and 3.28 ! 1.76 for PKW, and the frequency distributions fit very well the Poisson probability function with both mitogens. For 0.25 v g/mL and increasing BUdR concentrations, SCE/cell rates for pokeweed mitogen were significantly higher than those of PHA. At 5 4 g/mL of BUdR, the SCE/cell response was 8.68 ! 3.24 for PKW and 6.96 ! 3.45 for PHA, and the difference was statistically significant (P = 0.0001); for both mitogens the SCE/cell frequency distributions fit the Poisson probability function only by adopting a Poisson 'mixture' model, which takes into account the presence of two different subpopulations of cells. sister chromatid exchange / phytohemagglutinin / pokeweed / Poisson distribution / goat Résumé-Effets différentiels de la phytohémagglutinine et du phytolaque sur les SCE/ cellules très similaire : les valeurs moyennes ont été de 3,17 ::!: 1, 93 pour PHA et de 3, 28 ! 1, 76 pour PKW, et les distributions de fréquences se sont très bien ajustées à une loi de Poisson pour les deux mitogënes. À partir de 0,25 J1g/mL, les réponses à PKW ont toujours été supérieures à celles correspondant à PHA. À partir de 5 J1 g/mL de BUdR, la réponse SCE/ cellule a été de 8, 68 t 3, 24 pour PKW et 6, 96 ! 3,45 pour PHA et la différence a été significative. La distribution de fréquence pour l'ensemble des mitogènes s'est très bien ajustée à un mélangé de dezlx lois de Poisson, correspondant aux deux populations de cellules concernées par chaque mitogène. échange entre chromatides / phytohémagglutinine / phytolaque / comparaison / chèvres
-Karyotyping of a malformed Italian Friesian calf revealed one additional autosome in all the lymphocyte metaphases studied. This chromosome has been identified by G-banding using trypsin and giemsa staining (GTG) and R-banding by lateincorporation of BrdU and acridine orange staining (RBA) banding techniques, as probably being chromosome number 20, according to the International System for Cytogenetic Animals nomenclature (ISCNDA). The calf presented deformations of anterior and posterior legs, an opistotonous neck, lower brachygnathia, bilateral blindness and absence of external genitalia. The condition also appears to be associated with chromosome instability. autosomal trisomy / chromosome / anomaly / cattle Résumé-Une trisomie autosomique chez le bovin. L'analyse caryotypique d'un veau anormal de race Frisonne italienne a montré la présence d'un autosome surnuméraire dans toutes les métaphases étudiées. Le chromosome a été identifié par les techniques de bandes G et R ; il s'agit probablement du chromosome 20, en accord avec la nomenclature ISCNDA. Le veau présentait des déformations aux membres inférieurs et supérieurs, un cou opistotonique, une brachygnathie inférieure, une cécité bilatérale et l'absence d'organes génitaux externes. L'anomalie semble aussi associée à une fragilité des chromosomes. trisomie autosomique / chromosome / anomalie / bovin
Summary -A differential sister chromatid exchange (SCE)/cell response was observed between phytohemagglutinin (PHA) and pokeweed (PKW)
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