The large conducting arteries in vertebrates are composed of a specialized extracellular matrix designed to provide pulse dampening and reduce the work performed by the heart. The mix of matrix proteins determines the passive mechanical properties of the arterial wall 1

Determining arterial mechanical properties is important for understanding the work done by the heart and how it changes with cardiovascular disease. Ex vivo tests are necessary to apply various loads to the artery and obtain data to model and predict the behavior under any load. Most ex vivo tests are performed within 24 hours of dissection, so the tissue is still “alive”. For large elastic arteries, however, the passive mechanical behavior is attributed mostly to the very stable proteins, elastin and collagen. If the testing equipment fails, is in use, or is located at another facility, it would be useful to store the vessels and postpone the tests until the equipment is available. The goal of this study is to determine the effects of storage time on the mechanical behavior of the common carotid artery from adult mice. Each artery was tested after storage for 1–28 days in physiologic saline at 4°C. There were no significant effects of storage time on the arterial diameter or force at each pressure, but there were significant effects on the stretch ratio and stress at each pressure. The significant effects on the stretch ratio and stress were due to decreases in the unloaded dimensions with storage time, when measured from cut arterial rings. When the unloaded dimensions were measured instead from histology sections, there were no significant changes with storage time. We conclude that histology sections yield a more consistent measurement of the unloaded dimensions and that there are no significant changes in the mechanical behavior of mouse carotid artery with storage up to 28 days.

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Air curtains are created in open refrigerated vertical display cases for creating an invisible barrier between the cold air inside and the warm air outside the case. A systematic approach is developed to minimize the entrainment and infiltration of warm air into the case by optimizing the performance of an air curtain through adjusting pertinent flow parameters and case geometry. A modular display case was manufactured for the parametric studies. In this modular display case the geometry and flow parameters were changed and the infiltration rate was measured using a new technique, tracer gas method, which could be performed noticeably faster than the conventional methods. This apparatus is referred to as the proof-of-concept air curtain (POCAC). A matrix of all possible permutations was constructed with the infiltration rate being the outcome of this matrix. This matrix was populated by actual experimental measurements as well as using validated Computational Fluid Dynamics (CFD) computer programs as a tool to acquire better resolution of the input and output datasets. All the problem variables are referred to as the input vector of all parameters that can be altered, and the output is the infiltration rate. An artificial neural network (ANN) program was used to provide the linkage between the input vector (problem variables) and the problem outcome (infiltration). This program can also be used by industry as a tool to estimate the infiltration rate for all existing open vertical display cases.