The study was conducted on 90 serum samples of people with prostate diseases (G1-include 24 samples for patients with prostate cancer-PC , G2- include 24 samples for patients with benign prostatic hyperplasia-BPH and  22 samples for patients with prostatitis G3), compared with 20 samples for healthy individuals as control group. Prostate-specific antigen-PSA, human kallikrein-2-hK2, fibrinogen gamma-FGG, total anti-oxidant capacity-TAC, Vitamin D3-VD3. The results showed that the activity of PSA significantly elevated in G1 andG3 with no significant difference in G2, with significant increase in G1 for PSA and the hK2, FGG and TAC. so The results showed a significant increase in the group G2 in  human kallikrein-2 with no significant difference in G3 compared with control group, and a significant increase in FGG levels in the group G2 and G3 compared with control group with no significant difference between G3, G1 with a significant increase in the group G2 and G3 in TAC levels compared with the control group , with  significant decreased in VD3 levels in group G3 and no significant difference in groups G1, G2 compared with control group.