A series of new N-(substituted)-5-phenyl-1,3,4-thiadiazol-2-amine derivatives were synthesized under microwave irradiation and evaluated for their anti-inflammatory activity and in-silico (molecular docking studies) to recognize the hypothetical binding motif of the title compounds using VLifeMDS software. The binding mode of the title compounds has been proposed based on the docking studies. They have interesting pharmacophore that display a broad spectrum of biological activity. The 1,3,4-thiadiazole scaffold is an interesting building block that has been used to synthesize a variety of useful bioactive compounds. The present studies widen the scope of the brine shrimp model that may prove quite helpful as a preliminary screen to determine toxic properties. In Brine shrimp lethality bioassay, compounds produced dose dependent cytotoxicity effect to brine shrimp nauplii. Inflammation is a complex process, which is frequently associated with pain and involves occurrences such as the increase of vascular permeability, increase of protein denaturation, and membrane alteration. The pharmacological evaluation of 1,3,4-thiadiazole derivatives revealed that, among all the compounds screened compound code 3c were found to have promising anti-inflammatory activity.
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