Anthocyanins and phenolics of 10 blue honeysuckle (Lonicera caerulea L.) genotypes were characterized and quantified by HPLC-DAD. Peak assignments were confirmed by low-resolution electrospray mass spectrometry. Six anthocyanins were detected with the major peak identified as cyanidin 3-glucoside. Five additional anthocyanins were characterized as cyanidin 3,5-diglucoside, cyanidin 3-rutinoside, pelargonidin 3-glucoside, peonidin 3-glucoside, and peonidin 3-rutinoside. Four polyphenolics were identified as chlorogenic acid, neochlorogenic acid, quercetin 3-rutinoside, and quercetin 3-glucoside. Two additional unidentified phenolics were characterized as flavonol and hydroxycinnamic derivatives based on UV-vis spectra. Hydroxycinnamate levels ranged from 30.4 to 156.2 mg/100 g, whereas the flavonol content ranged from 12.6 to 32.8 mg/100 g. The L. caerulea subspecies boczkarnikovae contained the highest amounts of hydroxycinnamic derivatives and flavonols.
The U.S. Dept. of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository (NCGR), Corvallis, Ore., maintains Rubus germplasm representing worldwide diversity of the genus. Chromosome numbers were counted for 201 plants representing 124 taxa (species and varieties). There are new reports for 42 taxa, confirmation for 72 previously reported, and 10 counts for plants unidentified to species. The basic chromosome number was seven, and ploidy levels ranged from 2x to 12x.
`Gasaway' hazelnut (Corylus avellana L.) is highly resistant to eastern filbert blight caused by Anisogramma anomala (Peck) E. Muller. Progeny produced from controlled crosses of `Gasaway' with five susceptible genotypes and open pollination in a `DuChilly' orchard were planted in a diseased orchard and rated for symptom expression for 9 to 10 years. All progeny were found to segregate 50% resistant: 50% susceptible, indicating that `Gasaway' is heterozygous for a single dominant resistance gene.
Chromosome numbers were counted for 90 Rubus cultivars and selections maintained at the U.S. Dept. of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository, Corvallis, Ore. To my knowledge, 37 of the counts are new, including five that are corrections of previously published counts, 30 that are confirmations of numbers that were previously published but assumed from their parentage rather than actually counted, and 23 that are confirmations of previous counts. The basic number was 7, and 2n numbers ranged from 2x to 14x, including odd-ploids and aneuploids.
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