The aim of this study was to investigate the effect of supplementation of vitamin E, vitamin C, and zinc on the oxidative stress in burned children. In a prospective double-blind placebo controlled pilot study, 32 patients were randomized as no supplementation (n = 15) or antioxidant supplementation (n = 17) groups. Supplementation consisted of the antioxidant mixture of vitamin C (1.5 times upper intake level), vitamin E (1.35 times upper intake level), and zinc (2.0 times recommended dietary allowance) administered during 7 days starting on the second day of admittance into the hospital. Energy requirement was calculated by the Curreri equation, and protein input was 3.0 g/kg of ideal body mass index (percentile 50). Total antioxidant capacity of plasma and malondialdehyde were used to monitor oxidative stress. The time of wound healing was evaluated as the main clinical feature. Patients (age 54.2 +/- 48.9 months, 65.6% males), who exhibited 15.5 +/- 6.7% of total burn area, showed no differences in age and sex, when compared with controls. Intake of the administered antioxidants was obviously higher in treated subjects (P = .005), and serum differences were confirmed for vitamin E and C, but not for zinc (P = .180). There was a decrease in lipid peroxidation (malondialdehyde level) (P = .006) and an increase in vitamin E concentrations in the antioxidant supplementation group (P = .016). The time of wound healing was lower in the supplemented group (P < .001). The antioxidant supplementation through vitamin E and C and the mineral zinc apparently enhanced antioxidant protection against oxidative stress and allowed less time for wound healing.
Objective: To report the use of virtual reality (VR) in pain intensity during dressing change of two burned children hospitalized in a Burn Treatment Center (BTC) in Southern Brazil. Method: Case report on the use of VR during dressing change of two burned children hospitalized in a BTC, from May to July 2016. For assessing pain, a facial pain rating scale was applied at four times: just before the dressing, during the dressing without the use of VR, during the dressing with the VR, and after the use of VR. Results: The use of goggles was easy to apply and well-accepted by the children, and also had a relevant effect reducing pain. Conclusion: VR can become an important nonpharmacological method for treating pain in burned children. Descriptors: Virtual Reality Exposure Therapy; Burns; Pediatrics; Burn Units; Pain; Three-dimensional Imaging.RESUMO Objetivo: Relatar a utilização da Realidade Virtual (RV) na diminuição da intensidade dolorosa durante a troca de curativo de duas crianças queimadas internadas em um Centro de Tratamento ao Queimado (CTQ) do Sul do Brasil. Método: Relato de caso da utilização da RV durante a troca de curativos de duas crianças queimadas internadas em um CTQ, de maio a julho de 2016. Para avaliar a dor, foi utilizada escala numérica sobreposta à de faces, sendo aplicada em quatro momentos: imediatamente antes do curativo, durante o curativo sem uso da RV, durante o curativo com a RV e após a utilização da RV. Resultado: O uso dos óculos foi de fácil aplicação e bem aceito pelas crianças, além disso houce efeitos relevantes em relação à diminuição da dor. Conclusão: A RV pode se tornar um importante método não farmacológico no tratamento da dor em crianças queimadas. Descritores: Terapia de Exposição à Realidade Virtual; Queimaduras; Pediatria; Unidades de Queimados; Dor; Imagem Tridimensional. RESUMEN Objetivo: Relatar la utilización de la Realidad Virtual (RV) en disminución de intensidad del dolor durante cambio de vendajes de dos niños quemados, internados en un Centro de Tratamiento del Quemado (CTQ) del Sur de Brasil. Método: Relato de caso de utilización de RV durante cambio de vendajes de dos niños quemados internados en CTQ, de mayo a julio de 2016. Se evaluó el dolor utilizando escala numérica conjuntamente con la de faces, aplicándosela en cuatro momentos: inmediatamente antes del vendaje, durante el vendaje sin utilizar la RV, durante el vendaje utilizando RV y luego de la utilización de la RV. Resultado: El uso de los visores fue simple y bien recibido por los niños; además, hubo efectos relevantes en relación a la disminución del dolor. Conclusión: La RV puede constituir un importante método no farmacológico para el tratamiento del dolor en niños quemados.
New strategies for skin regeneration are needed in order to provide effective treatment for cutaneous wounds and disease. Mesenchymal stem cells (MSCs) are an attractive source of cells for tissue engineering because of their prolonged self-renewal capacity, multipotentiality, and ability to release active molecules important for tissue repair. In this paper, we show that human skin-derived mesenchymal stromal cells (SD-MSCs) display similar characteristics to the multipotent MSCs. We also evaluate their growth in a three-dimensional (3D) culture system with dermal substitutes (Integra and Pelnac). When cultured in monolayers, SD-MSCs expressed mesenchymal markers, such as CD105, Fibronectin, and α-SMA; and neural markers, such as Nestin and βIII-Tubulin; at transcriptional and/or protein level. Integra and Pelnac equally supported the adhesion, spread and growth of human SD-MSCs in 3D culture, maintaining the MSC characteristics and the expression of multilineage markers. Therefore, dermal substitutes support the growth of mesenchymal stromal cells from human skin, promising an effective tool for tissue engineering and regenerative technology.
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