Abstract. The combined effects of elevated pCO2 and temperature were investigated during an experimentally induced autumn phytoplankton bloom in vitro sampled from the western English Channel (WEC). A full factorial 36-day microcosm experiment was conducted under year 2100 predicted temperature (+4.5 ∘C) and pCO2 levels (800 µatm). Over the experimental period total phytoplankton biomass was significantly influenced by elevated pCO2. At the end of the experiment, biomass increased 6.5-fold under elevated pCO2 and 4.6-fold under elevated temperature relative to the ambient control. By contrast, the combined influence of elevated pCO2 and temperature had little effect on biomass relative to the control. Throughout the experiment in all treatments and in the control, the phytoplankton community structure shifted from dinoflagellates to nanophytoplankton . At the end of the experiment, under elevated pCO2 nanophytoplankton contributed 90 % of community biomass and was dominated by Phaeocystis spp. Under elevated temperature, nanophytoplankton comprised 85 % of the community biomass and was dominated by smaller nanoflagellates. In the control, larger nanoflagellates dominated whilst the smallest nanophytoplankton contribution was observed under combined elevated pCO2 and temperature (∼ 40 %). Under elevated pCO2, temperature and in the control there was a significant decrease in dinoflagellate biomass. Under the combined effects of elevated pCO2 and temperature, dinoflagellate biomass increased and was dominated by the harmful algal bloom (HAB) species, Prorocentrum cordatum. At the end of the experiment, chlorophyll a (Chl a) normalised maximum photosynthetic rates (PmB) increased > 6-fold under elevated pCO2 and > 3-fold under elevated temperature while no effect on PmB was observed when pCO2 and temperature were elevated simultaneously. The results suggest that future increases in temperature and pCO2 simultaneously do not appear to influence coastal phytoplankton productivity but significantly influence community composition during autumn in the WEC.

A 21-year time series of phytoplankton community structure was analysed in relation to Phaeocystis spp. to elucidate its contribution to the annual carbon budget at station L4 in the western English Channel (WEC).Between 1993-2014 Phaeocystis spp. contributed $4.6% of the annual phytoplankton carbon and during the March À May spring bloom, the mean Phaeocystis spp. biomass constituted 17% with a maximal contribution of 47% in 2001. Upper maximal weekly values above the time series mean ranged from 63 to 82% of the total phytoplankton carbon ($42-137 mg carbon (C) m À3 ) with significant interannual variability in Phaeocystis spp. Maximal biomass usually occurred by the end of April, although in some cases as early as mid-April (2007) and as late as late May (2013).The effects of elevated pCO 2 on the Phaeocystis spp. spring bloom were investigated during a fifteenday semi-continuous microcosm experiment. The phytoplankton community biomass was estimated at $160 mg C m À3 and was dominated by nanophytoplankton (40%, excluding Phaeocystis spp.), Phaeocystis spp. (30%) and cryptophytes (12%). The smaller fraction of the community biomass comprised picophytoplankton (9%), coccolithophores (3%), Synechococcus (3%), dinoflagellates (1.5%), ciliates (1%) and diatoms (0.5%). Over the experimental period, total biomass increased significantly by 90% to $305 mg C m À3 in the high CO 2 treatment while the ambient pCO 2 control showed no net gains. Phaeocystis spp. exhibited the greatest response to the high CO 2 treatment, increasing by 330%, from $50 mg C m À3 to over 200 mg C m À3 and contributing $70% of the total biomass.Taken together, the results of our microcosm experiment and analysis of the time series suggest that a future high CO 2 scenario may favour dominance of Phaeocystis spp. during the spring bloom. This has significant implications for the formation of hypoxic zones and the alteration of food web structure including inhibitory feeding effects and lowered fecundity in many copepod species.Crown

Diatoms are globally abundant algae that form extensive blooms in aquatic ecosystems. Certain bacteria behave antagonistically towards diatoms, killing or inhibiting their growth. Despite their crucial implications to diatom health and bloom control, insight of the prevalence and dynamics of antagonistic bacteria in nature is lacking. We report an ecosystem assessment of the diversity and seasonal patterns of bacterial antagonists of diatoms via regular plaque-assay sampling in the Western English Channel (WEC), where diatoms frequently bloom. Unexpectedly, peaks in antagonist detection did not occur during characteristic spring blooms, but coincided with a winter bloom of Coscinodiscus, suggesting bacterial pathogens likely influence distinct diatom host populations. We isolated multiple antagonists, spanning 4 classes and 10 bacterial orders. Many species had no prior reports of pathogenicity towards diatoms, and we verified diatom growth inhibitory effects of 8 isolates. In all cases tested, pathogenicity was activated by pre-exposure to diatom organic matter. Discovery of widespread cryptic antagonistic activity evident under specific conditions, indicates that bacterial pathogenicity towards diatoms is more prevalent than previously recognised. Finally, mining Tara Oceans data revealed the global biogeography of WEC antagonists and co-occurrence patterns with diatom hosts. Our study indicates that multiple, diverse antagonistic bacteria have potential to impact diatom growth and bloom dynamics in marine waters globally.

<p><strong>Abstract.</strong> The combined effects of elevated pCO<sub>2</sub> and temperature were investigated during an autumn phytoplankton bloom in the Western English Channel (WEC). A full factorial 36-day microcosm experiment was conducted under year 2100 predicted temperature (+4.5 °C) and pCO<sub>2</sub> levels (800 μatm). The starting phytoplankton community biomass was 110.2 (±5.7 sd) mg carbon (C) m<sup>−3</sup> and was dominated by dinoflagellates (~ 50 %) with smaller contributions from nanophytoplankton (~ 13 %), cryptophytes (~ 11 %)and diatoms (~ 9 %). Over the experimental period total biomass was significantly increased by elevated pCO<sub>2</sub> (20-fold increase) and elevated temperature (15-fold increase). In contrast, the combined influence of these two factors had little effect on biomass relative to the ambient control. The phytoplankton community structure shifted from dinoflagellates to nanophytoplankton at the end of the experiment in all treatments. Under elevated pCO<sub>2</sub> nanophytoplankton contributed 90% of community biomass and was dominated by <i>Phaeocystis</i> spp., while under elevated temperature nanophytoplankton contributed 85 % of the community biomass and was dominated by smaller nano-flagellates. Under ambient conditions larger nano-flagellates dominated while the smallest nanophytoplankton contribution was observed under combined elevated pCO<sub>2</sub> and temperature (~ 40 %). Dinoflagellate biomass declined significantly under the individual influences of elevated pCO<sub>2</sub>, temperature and ambient conditions. Under the combined effects of elevated pCO<sub>2</sub> and temperature, dinoflagellate biomass almost doubled from the starting biomass and there was a 30-fold increase in the harmful algal bloom (HAB) species, <i>Prorocentrum cordatum</i>. Chlorophyll a normalised maximum photosynthetic rates (P<sup>B</sup><sub>m</sub>) increased > 6-fold under elevated pCO<sub>2</sub> and > 3-fold under elevated temperature while no effect on P<sup>B</sup><sub>m</sub> was observed when pCO<sub>2</sub> and temperature were elevated simultaneously. The results suggest that future increases in temperature and pCO<sub>2</sub> do not appear to influence coastal phytoplankton productivity during autumn in the WEC which would have a negative feedback on atmospheric CO<sub>2</sub>.</p>