The alpha-subunit of MDH has an eight-fold radial symmetry, with its eight beta-sheets stabilized by a novel tryptophan docking motif. The PQQ in the active site is held in place by a coplanar tryptophan and by a novel disulphide ring formed between adjacent cysteines which are bonded by an unusual non-planar trans peptide bond. One of the carbonyl oxygens of PQQ is bonded to the Ca2+, probably facilitating attack on the substrate, and the other carbonyl oxygen is out of the plane of the ring, confirming the presence of the predicted free-radical semiquinone form of the prosthetic group.

The complete genome sequence of Helicobacter pylori has revealed the presence of a novel set of chemotaxis genes including three cheV paralogues. CheV is a bi-functional protein, the N-terminal domain being homologous to the signalling-complex linker protein CheW, while the C-terminal domain is homologous to the response-regulator CheY, but its precise function in chemotaxis is unknown. In this study, each of the three cheV paralogues were insertionally inactivated in strain 26695 to determine their importance in the chemotactic signal-transduction pathway of H. pylori. Mutation of HP0019 (cheV1) had a severe inhibitory effect on chemotaxis, as determined by a swarm-plate assay. In contrast, strains carrying single mutations in either cheV2 (HP0616) or cheV3 (HP0393) displayed wild-type swarming behaviour, as did a cheV2/cheV3 double mutant. However, expression of the cheV2 or cheV3 genes in Escherichia coli resulted in an inhibition of chemotaxis in a wild-type strain, indicating their role in chemotaxis, although these genes were unable to complement isogenic E. coli cheW or cheY mutants. The product of cheV2/HP0616 was overexpressed in E. coli and purified to homogeneity. Protein fluorescence quenching experiments showed that CheV2 was capable of binding acetyl phosphate, a small-molecule phosphodonor. The measured K m for acetyl phosphate was 21 mM. It is concluded that in the absence of a cheZ gene, the CheV proteins could act as phosphate sinks to control the cellular level of phospho-CheY in H. pylori. However, only CheV1 was critical for chemotaxis, indicating a specific role distinct from the other paralogues in the signal-transduction pathway. Significantly, none of the CheV proteins could substitute for the loss of CheW, as an H. pylori cheW null mutant was nonchemotactic.

No abstract

The quinoprotein methanol dehydrogenase (MDH) contains a Ca2+ ion at the active site. Ca(2-)-free enzyme (from a processing mutant) was used to obtain enzyme containing Sr2+ or Ba2+, the Ba(2+)-MDH being the first enzyme to be described in which a Ba2+ ion functions at the active site. The activation energy for oxidation of methanol by Ba(2+)-MDH is less than half that of the reaction catalysed by Ca(2+)-MDH (a difference of 21.4 kJ/mol), and the Vmax value is 2-fold higher. The affinities of Ba(2+)-MDH for substrate and activator are very much less than those of Ca(2+)-MDH; the Km for methanol is 3.5 mM (compared with 3 microM) and the KA for ammonia is 52 mM (compared with 2 mM). The different activity of Ba(2+)-MDH is probably due to a change in the conformation of the active site, leading to a decrease in the free energy of substrate binding and hence a decrease in activation energy. The kinetic model for Ba(2+)-MDH with respect to substrate and activator is consistent with previous models for Ca(2+)-MDH. The pronounced deuterium isotope effect (6.0-7.6) is influenced by ammonia, and is consistent with activation of the pyrroloquinoline quinone reduction step by ammonia. Because of its low affinity for substrates, it is possible to prepare the oxidized form of Ba(2+)-MDH. No spectral intermediates could be detected during reduction by added substrate, and so it is not possible to distinguish between those mechanisms involving covalent substrate addition and those involving only hydride transfer.

Photo-excitation of membrane-bound Rhodobacter sphaeroides reaction centres containing the mutation Ala M260 to Trp (AM260W) resulted in the accumulation of a radical pair state involving the photo-oxidised primary electron donor (P). This state had a lifetime of hundreds of milliseconds and its formation was inhibited by stigmatellin. The absence of the Q A ubiquinone in the AM260W reaction centre suggests that this long-lived radical pair state is P + Q 3 B , although the exact reduction/protonation state of the Q B quinone remains to be con¢rmed. The blockage of active branch (A-branch) electron transfer by the AM260W mutation implies that this P + Q 3 B state is formed by electron transfer along the so-called inactive branch (B-branch) of reaction centre cofactors. We discuss how further mutations may a¡ect the yield of the P + Q 3 B state, including a double alanine mutation (EL212A/DL213A) that probably has a direct e¡ect on the e⁄ciency of the low yield electron transfer step from the anion of the B-branch bacteriopheophytin (H 3 B ) to the Q B ubiquinone.