1999
DOI: 10.1023/a:1006111321083
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Cited by 49 publications
(56 citation statements)
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References 73 publications
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“…This long lifetime indicates stabilization of QB -by this double mutation, in accord with previous observations [35] . In a photoelectrochemical cell, with a mechanism for effective reduction of P + by the working electrode using cyt c as a "wire", such stabilization would be expected to result in photoaccumulation of RCs initially in the state PQB -and, following a second photoexcitation and in the absence of double reduction of QB, the state PQA -QB -.…”
Section: Discussionsupporting
confidence: 92%
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“…This long lifetime indicates stabilization of QB -by this double mutation, in accord with previous observations [35] . In a photoelectrochemical cell, with a mechanism for effective reduction of P + by the working electrode using cyt c as a "wire", such stabilization would be expected to result in photoaccumulation of RCs initially in the state PQB -and, following a second photoexcitation and in the absence of double reduction of QB, the state PQA -QB -.…”
Section: Discussionsupporting
confidence: 92%
“…This mutation causes the RC to assemble without a QA ubiquinone, halting charge separation at the P + HA -state. The AM260W RC has been characterized by a range of spectroscopic techniques [35,44,45], and a 2.1 Å resolution X-ray crystal structure has been determined that showed a lack of a ubiquinone at the QA site and a QB site occupied by UQ10 [36]. In the present study, photoexcitation of purified AM260W RCs failed to produce a change in 865 nm absorbance, indicating a lack of P + formation on a millisecond time scale (Figure 3, black).…”
Section: Qa Binding Site Blocking Mutant Rc Am260wmentioning
confidence: 51%
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“…This residue was mutated to tryptophan with a view to causing exclusion of the Q A ubiquinone and so preventing light-driven transmembrane electron transfer in the reaction center. Spectroscopic studies, a full account of which has been given recently (52), showed that the AM260W reaction center does not possess Q A function, and in particular picosecond time-scale transient absorption spectroscopy showed that light-driven electron transfer is blocked at the state P ϩ H L Ϫ . To characterize further the AM260W reaction center, the complex was purified and crystallized, and x-ray diffraction data were collected according to the procedures described in Methods.…”
Section: Resultsmentioning
confidence: 99%