The M-type phospholipase A2 receptor (PLA2R1) is a member
of the C-type lectin superfamily and can internalize secreted phospholipase
A2 (sPLA2) via endocytosis in non-cancer cells.
sPLA2 itself was recently shown to be overexpressed in
prostate tumors and to be a possible mediator of metastasis; however,
little is known about the expression of PLA2R1 or its function in
prostate cancers. Thus, we examined PLA2R1 expression in primary prostate
cells (PCS-440-010) and human prostate cancer cells (LNCaP, DU-145,
and PC-3), and we determined the effect of PLA2R1 knockdown on cytotoxicity
induced by free or liposome-encapsulated chemotherapeutics. Immunoblot
analysis demonstrated that the expression of PLA2R1 was higher in
prostate cancer cells compared to that in primary prostate cells.
Knockdown of PLA2R1 expression in PC-3 cells using shRNA increased
cell proliferation and did not affect the toxicity of cisplatin, doxorubicin
(Dox), and docetaxel. In contrast, PLA2R1 knockdown increased the
in vitro toxicity of Dox encapsulated in sPLA2 responsive
liposomes (SPRL) and correlated with increased Dox and SPRL uptake.
Knockdown of PLA2R1 also increased the expression of Group IIA and
X sPLA2. These data show the novel findings that PLA2R1
is expressed in prostate cancer cells, that PLA2R1 expression alters
cell proliferation, and that PLA2R1 modulates the behavior of liposome-based
nanoparticles. Furthermore, these studies suggest that PLA2R1 may
represent a novel molecular target for controlling tumor growth or
modulating delivery of lipid-based nanomedicines.
Recent studies suggest that glypican-1 (GPC-1) is a biomarker for prostate cancer, but there are few studies elucidating the role of GPC-1 in prostate cancer progression. We observed high expression of GPC-1 in more aggressive prostate cancer cell lines such as PC-3 and DU-145. While inhibition of GPC-1 expression in PC-3 cells decreased cell growth and migration
in vitro
, it surprisingly increased cell proliferation and migration in DU-145 cells, suggesting that the role of GPC-1 is cell type-dependent. Further, GPC-1 inhibition increased PC-3 tumor size in NCr nude mice xenografts. We hypothesized that the discrepancy between the
in vitro
and
in vivo
data is mediated by stromal cells in the tumor microenvironment. Thus, we tested the effect of tumor conditioned media (TCM) on gene expression in human mesenchymal stem cells and fibroblasts. Treatment of stromal cells with TCM from PC-3 cells transfected with GPC-1 shRNA increased the expression of migration markers, endocrine/paracrine biomolecules, and extracellular matrix components. Additionally, the decreased cell growth in GPC-1 knockdown PC-3 cells was rescued by coculturing with stromal cells. These data demonstrate the paradoxical role that GPC-1 plays in prostate cancer cell growth by interacting with stromal cells and through ECM remodeling and endocrine/paracrine signaling.
Doxorubicin is associated with "chemobrain," a long-term cancer related cognitive impairment. Doxorubicin treatment results in hippocampus-dependent learning and memory deficits. Impairment in hippocampal long-term potentiation is accompanied with reduced functionality of glutamate subtype AMPA receptor. Dysregulation of CaMKII, BDNF, ERK, and AKT protein signaling leading to learning and memory impairment.
In order to evaluate the effectiveness of L: -lactate dehydrogenase (LDH) from rabbit muscle as a regenerative catalyst of the biologically important cofactor nicotinamide adenine dinucleotide (NAD), the kinetics over broad concentrations were studied to develop a suitable kinetic rate expression. Despite robust literature describing the intricate complexations, the mammalian rabbit muscle LDH lacks a quantitative kinetic rate expression accounting for simultaneous inhibition parameters, specifically at high pyruvate concentrations. Product inhibition by L: -lactate was observed to reduce activity at concentrations greater than 25 mM, while expected substrate inhibition by pyruvate was significant above 4.3 mM concentration. The combined effect of ternary and binary complexes of pyruvate and the coenzymes led to experimental rates as little as a third of expected activity. The convenience of the statistical software package JMP allowed for effective determination of experimental kinetic constants and simplification to a suitable rate expression: [formula: see text] where the last three terms represent the inhibition complex terms for lactate, pyruvate, and pyruvate-NAD, respectively. The corresponding values of K (I-Lac), K (I-Pyr), and K (I-Pyr-NAD) for rabbit muscle LDH are 487.33 mM(-1) and 29.91 mM and 97.47 mM at 22 °C and pH 7.8.
Pre-clinical monitoring of tumor growth and identification of distal metastasis requires a balance between accuracy and expediency. Bioluminescence imaging (BLI) is often used to track tumor growth but is primarily limited to planar 2-dimensional (2D) imaging. Consistent subject placement within a standard top-mounted, single-detector small animal imager is vital to reducing variability in repeated same-animal measures over time. Here, we describe a method for tracking tumor development using a multi-angle BLI and photo-acoustic workflow. We correlate serial caliper measurements and 2D BLI to 360° BLI and photo-acoustic datasets for the same animals. Full 360° BLI showed improved correlations with both volumes obtained from caliper measurements and photo-acoustic segmentation, as compared to planar BLI. We also determined segmented tumor volumes from photo-acoustic datasets more accurately reflects true excised tumors’ volumes compared to caliper measurements. Our results demonstrate the distinct advantages of both 360° surface mapping by BLI and photo-acoustic methodologies for non-invasive tracking of tumor growth in pre-clinical academic settings. Furthermore, our design is fully implementable in all top-mounted, single-detector imagers, thereby providing the opportunity to shift the paradigm away from planar BLI into rapid BLI tomography applications.
In order to evaluate the effectiveness of aldehyde dehydrogenase (ALDH) from Saccharomyces cerevisiae as a catalyst for the conversion of acetaldehyde into its physiologically and biologically less toxic acetate, the kinetics over broad concentrations were studied to develop a suitable kinetic rate expression. Even with literature accounts of the binding complexations, the yeast ALDH currently lacks a quantitative kinetic rate expression accounting for simultaneous inhibition parameters under higher acetaldehyde concentrations. Both substrate acetaldehyde and product NADH were observed as individual sources of inhibition with the combined effect of a ternary complex of acetaldehyde and the coenzyme leading to experimental rates as little as an eighth of the expected activity. Furthermore, the onset and strength of inhibition from each component were directly affected by the concentration of the co-substrate NAD. While acetaldehyde inhibition of ALDH is initiated below concentrations of 0.05 mM in the presence of 0.5 mM NAD or less, the acetaldehyde inhibition onset shifts to 0.2 mM with as much as 1.6 mM NAD. The convenience of the statistical software package JMP allowed for effective determination of experimental kinetic constants and simplification to a suitable rate expression: v = Vmax(AB)/(K(ia)K(b) + K(b)A + K(a)B + AB + B(2)/K(I-Ald) + B(2)Q/K(I-Ald-NADH) + BQ/K(I-NADH)) where the last three terms represent the inhibition complex terms for acetaldehyde, acetaldehyde-NADH, and NADH, respectively. The corresponding values of K(I-Ald), K(I-Ald-NADH), and K(I-NADH) for yeast ALDH are 2.55, 0.0269, and 0.162 mM at 22 °C and pH 7.8.
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