Migratory barriers, habitat loss, entrainment in irrigation canals, and overexploitation, especially at times of aggregation, have been suggested to explain the failure of Yellowstone River saugers Sander canadensis to return to historical abundances after a late-1980s decline that was attributed to drought. These factors are thought to affect saugers throughout their range and migratory large-river fishes in general. We characterized the seasonal movement patterns, habitat use, and aggregation of saugers and estimated movement, exploitation, and irrigation canal entrainment rates to test these hypotheses. Saugers aggregated near spawning areas in spring and subsequently dispersed 5-350 km to upstream home locations, where they remained for the rest of the year. Upstream movement was not overtly restricted by low-head diversion dams. During the spawning period, terrace and bluff pools, which are unique geomorphic units associated with bedrock and boulder substrate, were positively selected, while all other habitat types were avoided. Tributary spawning was rare. After moving to home locations, saugers used most habitat types in proportion to their availability but selected reaches in specific geologic types that allowed formation of deep, long pools. Exploitation occurred primarily in early spring and late autumn, was low annually (18.6%), and was not related to aggregation. Annual survival was high (70.4%). Entrainment in one irrigation diversion accounted for more than half of all nonfishing mortality. Therefore, habitat loss and overexploitation probably did not prevent sauger recovery, as the absence of migratory barriers allowed adult saugers unrestricted access to widely separated and diverse habitats and did not induce artificial aggregation. In other systems, population declines attributed to overexploitation during periods of aggregation may therefore have been caused fundamentally by migration barriers that created artificial aggregations.

Prior to anthropogenic modifications, the historic Missouri River provided ecological conditions suitable for reproduction, growth, and survival of pallid sturgeon Scaphirhynchus albus. However, little information is available to discern whether altered conditions in the contemporary Missouri River are suitable for feeding, growth and survival of endangered pallid sturgeon during the early life stages. In 2004 and 2007, nearly 600 000 pallid sturgeon free embryos and larvae were released in the upper Missouri River and survivors from these releases were collected during 2004-2010 to quantify natural growth rates and diet composition. Based on genetic analysis and known-age at release (1-17 days post-hatch, dph), age at capture (dph, years) could be determined for each survivor. Totals of 23 and 28 survivors from the 2004 and 2007 releases, respectively, were sampled. Growth of pallid sturgeon was rapid (1.91 mm day )1 ) during the initial 13-48 dph, then slowed as fish approached maximum length (120-140 mm) towards the end of the first growing season. The diet of youngof-year pallid sturgeon was comprised of Diptera larvae, Diptera pupae, and Ephemeroptera nymphs. Growth of pallid sturgeon from ages 1-6 years was about 48.0 mm year )1 . This study provides the first assessment of natural growth and diet of young pallid sturgeon in the wild. Results depict pallid sturgeon growth trajectories that may be expected for naturally produced wild stocks under contemporary habitat conditions in the Missouri River and Yellowstone River.

Summary We synthesized wild and stocked pallid sturgeon Scaphirhynchus albus capture data collected in four recovery areas of the Missouri River during 1998–2007, providing the first basin‐wide analysis of size structure, growth, and condition. Proportional size distribution (PSD) values ranged from 20 to 33 and were indicative of past stockings given the continued lack of natural recruitment. A new weight‐length regression derived from 2268 captured wild (8%), hatchery‐stocked (75%), and unknown origin (16%) pallid sturgeon had a significantly lower slope and intercept from a previously published model that used only 214 wild fish and a truncated size range. Relative condition (Kn) declined after stocking throughout the basin but stabilized at 0.94 within 3 years. Spatially, Kn of juvenile pallid sturgeon (330–629 mm) was generally highest in the reaches of the Missouri River with large tributaries. In accordance with the latitudinal counter gradient growth hypothesis, similar absolute growth increments (both length and weight) of tagged age‐1 pallid sturgeon in the Upper and Lower Missouri River indicated upstream fish grew at faster rates given the 1.3 fold difference in growing season length. From North to South, von Bertalanffy growth coefficients (k) showed a latitudinal increase while L∞ decreased, providing additional support of the latitudinal counter gradient growth hypothesis. However, growth rates of tagged juvenile pallid sturgeon aged 2–6 years were highest at the two most downstream reaches showing an increased influence of growing season length. In the Missouri River, pallid sturgeon (≤ ages 1–9 or 10 years) exhibited linear growth in the two most upstream reaches (Montana and North Dakota), exhibited logistic growth in the inter‐reservoir reach in South Dakota/Nebraska while a power function best described growth downstream of the lowermost dam (Nebraska, Iowa, Kansas, and Missouri). Differing growth models among reaches highlights the confounding affect of habitat fragmentation by dams in the Missouri River on growth as well as potential latitudinal affects on sexual maturation. Relative condition and growth of pallid sturgeon appears adequate throughout the Missouri River indicating success of past stocking efforts to forestall extirpation.

Worldwide, freshwater ecosystems are threatened by invasive species, resulting in adverse effects on infrastructure, economy, recreation, and native aquatic communities. In stream settings, chemical piscicides can be an effective tool for eradicating invasive fishes. However, chemical treatments are expensive and time consuming, and they do not discriminate between invasive and native species in a system. Therefore, managers would ideally limit treatment to only the area occupied by the invasive species. Because traditional survey methods may not accurately detect individuals in low abundance (e.g., at the edge of their distribution, or following an eradication effort), chemical treatments may be applied more broadly and more often than is necessary to ensure complete coverage. Furthermore, inadequate posttreatment sampling can fail to detect survivors of a treatment. As a result, managers may erroneously conclude that eradication was successful, leaving the ecosystem vulnerable to reestablishment by the invader. More sensitive sampling tools should allow for more precise definition of the treatment area and more accurate evaluation of project success. This would reduce project costs and overall effects on native species. Here, we illustrate how environmental DNA (eDNA) sampling addressed these challenges through three case studies, each of which used eDNA sampling to inform the removal of Brook Trout Salvelinus fontinalis in small streams. We found that eDNA methods can be informative throughout all stages of eradication projects in stream settings. It can assist with delimiting the population