In response to recommendations to redefine statistical significance to p ≤ .005, we propose that researchers should transparently report and justify all choices they make when designing a study, including the alpha level.

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Transmembrane protein transporters possessing binding sites for ions, toxins, pharmaceutical drugs, and other molecules constitute excellent candidates for developing sensitive and selective biosensing devices. Their attractiveness for analytical purposes is enhanced by the intrinsic amplification capabilities shown when the binding event leads to major changes in the transportation of ions or molecules other than the analyte itself. The large-scale implementation of such transmembrane proteins in biosensing devices is limited by the difficulties encountered in inserting functional transporters into artificial bilayer lipid membranes and by the limitations in understanding and exploiting the changes induced by the interaction with the analyte for sensing purposes. Here, we show that lysenin, a pore-forming toxin extracted from earthworm Eisenia foetida, which inserts stable and large conductance channels into artificial bilayer lipid membranes, functions as a multivalent ion-sensing device. The analytical response consists of concentration and ionic-species-dependent macroscopic conductance inhibition most probably linked to a ligand-induced gating mechanism. Multivalent ion removal by chelation or precipitation restores, in most cases, the initial conductance and demonstrates reversibility. Changes in lipid bilayer membrane compositions leading to the absence of voltage-induced gating do not affect the analytical response to multivalent ions. Microscopic current analysis performed on individual lysenin channels in the presence of Cu(2+) revealed complex open-closed transitions characterized by unstable intermediate sub-conducting states. Lysenin channels provide an analytical tool with a built-in sensing mechanism for inorganic and organic multivalent ions, and the excellent stability in an artificial environment recommend lysenin as a potential candidate for single-molecule detection and analysis.

Dijksterhuis and van Knippenberg (1998) reported that participants primed with a category associated with intelligence ("professor") subsequently performed 13% better on a trivia test than participants primed with a category associated with a lack of intelligence ("soccer hooligans"). In two unpublished replications of this study designed to verify the appropriate testing procedures, Dijksterhuis, van Knippenberg, and Holland observed a smaller difference between conditions (2%-3%) as well as a gender difference: Men showed the effect (9.3% and 7.6%), but women did not (0.3% and -0.3%). The procedure used in those replications served as the basis for this multilab Registered Replication Report. A total of 40 laboratories collected data for this project, and 23 of these laboratories met all inclusion criteria. Here we report the meta-analytic results for those 23 direct replications (total N = 4,493), which tested whether performance on a 30-item general-knowledge trivia task differed between these two priming conditions (results of supplementary analyses of the data from all 40 labs, N = 6,454, are also reported). We observed no overall difference in trivia performance between participants primed with the "professor" category and those primed with the "hooligan" category (0.14%) and no moderation by gender.

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Several theories have been proposed to account for variation in the intensity of life regrets. Variables hypothesized to affect the intensity of regret include: whether the regretted decision was an action or an inaction, the degree to which the decision was justified, and the life domain of the regret. No previous study has compared the effects of these key predictors in a single model in order to identify which are most strongly associated with the intensity of life regret. In this study, respondents (N = 500) to a postal survey answered questions concerning the nature of their greatest life regret. A Bayesian regression analysis suggested that regret intensity was greater for—in order of importance—decisions that breached participants’ personal life rules, decisions in social life domains than non-social domains, and decisions that lacked an explicit justification. Although regrets of inaction were more frequent than regrets of action, regrets relating to actions were slightly more intense.

A number of previous studies have reported a positive relationship between ambient temperature and the incidence of violent crimes such as assault. This has led some authors to suggest that anthropogenic climate change may result in an increase in violent crime rates. In this study, we report an investigation of the relationship between temperature and assault incidence in New Zealand. Both police data listing recorded assaults as well as data from the Ministry of Health listing hospitalisations due to assault were examined. Geographical, seasonal, and irregular daily variation in temperature were all positively related to the incidence of assault, although only the effect of irregular variation in temperature was robust to controls for plausible confounds. The estimated effect of irregular daily variation in temperature was approximately 1.5 % extra recorded assaults for each 1°C increase in temperature. It remains difficult, however, to make accurate predictions about future assault rates in a warming world. For example, humans may react to sustained changes in climate in ways that differ markedly from their reaction to short-term variation in temperature. Climate change may also affect rates of violence via mechanisms other than those that currently drive the relationship between temperature and violence. Furthermore, assault rates may continue to change in response to factors unrelated to climate change, such as those responsible for the long-term historical decline in human violence.