Reversible lysine acetylation is one of the most widely distributed post‐translational modifications; it is involved in a variety of biological processes and can be found in all three domains of life. Acetyltransferases and deacetylases work coordinately to control levels of protein acetylation. In this work, we applied the genetic code expansion strategy to site‐specifically incorporate Nε‐thioacetyl‐l‐lysine (TAcK) as an analog of Nε‐acetyl‐l‐lysine (AcK) into green fluorescent protein and malate dehydrogenase in Escherichia coli. We showed that TAcK could serve as an ideal functional mimic for AcK. It could also resist the bacterial sirtuin‐type deacetylase CobB. Thus, genetic incorporation of TAcK as a non‐deacetylatable analog of AcK into proteins will facilitate in vivo studies of protein acetylation.
cyclohexane derivativescyclohexane derivatives Q 0040
-094Intramolecular Competitive Ireland Claisen Rearrangements: Stereoselective Synthesis of Alkylidene Cyclohexenes.-The Ireland Claisen rearrangement of in situ generated bis-allyl ketene acetals is investigated. Thus, treatment of the esters (I) with triisopropylsilyl triflate affords an E/Z-mixture of alkylidene cyclohexenes (II) and (III) after desilylation. Introduction of a substituent on the endo double bond results in reversal of the stereoselectivity providing the E-diene (V) exclusively. Furthermore, rearrangement of the (R)-carvone-derived substrate (VI) yields the E-diene (VII) with satisfying chirality transfer by 1,6-asymmetric induction.-(ZHANG, X.; MCINTOSH, M. A.; Tetrahedron Lett. 39 (1998) 39, 7043-7046; Dep. Chem.
Harvard Medical School we believe that simple, focused, universally accessible resources like Organiclinks and Chembiolinks provide an easy way for communities of scientists of similar interests to get connected. We are confident that these resources will continue to enable scientists to expand their networks, and hope that they continue to inspire the creation of similar directories in other areas of science.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.