No abstract

ResumoO Role-Playing Game (RPG) é definido como um jogo de interpretação de papéis, no qual os participantes podem jogar uns contra os outros, ou trabalharem juntos em prol de um objetivo. Assim, elaborou-se um jogo de RPG para trabalhar o conteúdo de Imunologia com crianças e adolescentes (5 a a 8 a série) onde as batalhas medievais, envolvendo criaturas fantásticas, se associam a elementos que compõem o Sistema Imunológico, como um xamã que representa um linfócito T. O mestre, neste caso o professor, guiará os participantes para ensinar o conteúdo de forma lúdica, ao longo de uma "guerra" dentro de uma pessoa. Os alunos controlarão os personagens responsáveis por atacar e defender o corpo (agentes infecciosos e glóbulos brancos, respectivamente) dispondo de um dado para a rolagem de suas ações no jogo. Como resultado, espera-se com este trabalho que o conteúdo de Imunologia seja fixado mais facilmente, de forma agradável e divertida.Palavras-chave: método lúdico; Sistema Imunológico; ensino. AbstractRPG is defined as a Role-Playing Game, in which participants can play against each other, or work together towards a objective. Thereby, an RPG game was developed to work on the content of Immunology with children and adolescents (5th to 8th grade) where medieval battles, involving fantastic creatures, are associated with elements that make up the Immune System, such as a shaman representing an T lymphocyte. To teach Immunology through the RPG is primarily needed a master, will guide the participants to teach the content in a playful way, throughout a "war" within a person. The students who will control the characters responsible for attacking and defending the body (infectious agents and white blood cells, respectively) having a dice for the scrolling of their actions in the game. As a result, it is expected with this work that Immunology content will be more easily fixed in a fun and enjoyable way.

We are presenting the complete mitogenomes of eight fish species/cytotypes from Neotropical region belonging to the Astyanax and Psalidodon genus: A. aeneus, A. altiparanae, P. fasciatus (from two locations - Upper Parana and Sao Francisco river basins), A. lacustris, P. rivularis (two cytotypes) and P. rioparanaibano. We perform the whole-genome sequencing for six of these species in a Novaseq 6000 - by Illumina, meanwhile two genomes were assembled from raw data available in databases. Plus, we reassembled and annotated the mitochondrial genomes for A. mexicanus and P. paranae, both already described and with raw data available online. All the genomes presented the same organization, with 13 protein-coding genes, 22 tRNA genes and two rRNA genes. Aiming to contribute to the understanding of the several cryptic species complexes and phylogeny of the genus, we perform Bayesian analysis using the 13 protein-coding genes from these species, plus Deuterodon giton and using a Brycon species as outgroup.