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Apoptosis, autophagy and necrosis are three distinct functional types of the mammalian cell death network. All of them are characterized by a number of cell's morphological changes. The inappropriate induction of cell death is involved in the pathogenesis of a number of diseases.Pathogenesis of inflammatory bowel diseases (ulcerative colitis, Crohn's disease) includes an abnormal immunological response to disturbed intestinal microflora. One of the most important reason in pathogenesis of chronic inflammatory disease and subsequent multiple organ pathology is a barrier function of the gut, regulating cellular viability. Recent findings have begun to explain the mechanisms by which intestinal epithelial cells are able to survive in such an environment and how loss of normal regulatory processes may lead to inflammatory bowel disease (IBD).This review focuses on the regulation of biological pathways in development and homeostasis in IBD. Better understanding of the physiological functions of biological pathways and their influence on inflammation, immunity, and barrier function will simplify our expertice of homeostasis in the gastrointestinal tract and in upgrading diagnosis and treatment.

Various types of stem cells exist, each with their own advantages and disadvantages. Considering the current available evidence, important preclinical and clinical studies regarding the therapeutic potential of stem cells, stem cell therapy might be the important strategy for tissue repair. The development of stem cell therapy for tissue repair has primarily relied on stem cells, especially mesenchymal stem cells. Multilineage differentiation into all of the described cells are considered as important candidates for a range of diseases like neurological diseases, cardiovascular diseases, gastrointestinal cancer and genetic defects, as well as for acute and chronic wounds healing and pharmaceutical treatment. We review the properties and multipotency of stem cells and their differentiation potential, once cultured under specific growth conditions, for use in cell-based therapies and functional tissue replacement.

Purpose: Toxin B (TcdB) is a product of the bacteria Clostridium difficile, and can trigger apoptosis or necrosis. In the present manuscript, we proposed that TcdBVPI and TcdB8864 would cause morphological changes in cultured cells that are characteristic of autophagy, a constitutive cellular event closely linked with apoptosis or necrosis. Methods: We examined an in vitro model of cultured HT-29 cells to determine the occurrence of autophagy. The cells were incubated separately with TcdBVPI and TcdB8864 for 2 to 24 hours, and then examined using transmission electron microscopy. Results: In HT-29 cells, ultrastructural changes were observed following 8 hours of treatment with TcdBVPI and TcdB8864. Upon exposure to both toxins, complex changes occurred that affected the cellular framework, including the dilated regions of the granular endoplasmic reticulum, the Golgi apparatus, as well as electron-lucent and electron-dense autophagosome-like structures. Additionally, lipid droplets were present in the cytoplasm of HT-29 cells. Conclusion: Our findings indicate that TcdBVPI and TcdB8864 indeed induce ultrastructural changes in HT-29 cells. The presence of these autophagic structures in the cytoplasm of the HT-29 cells suggests that autophagy may be induced during treatment by both toxins. Therefore, this effect could represent an important protective mechanism for cell survival.

IzvlečekZgornja mezenterična arterija, druga sprednja veja trebušne aorte, oskrbuje spodnji del dvanajstnika, glavo in odrastek trebušne slinavke, tanko in debelo črevo do sredine prečnega dela debelega črevesa. Veje zgornje mezenterične arterije so: spodnja sprednja in spodnja zadnja pankreatikoduodenalna arterija, srednja količna arterija, desna količna arterija, ileokolična arterija in arterije vitega in tešče-ga črevesja. Anatomske variacije vej zgornje mezenterične arterije so pogoste. Vzroki za anatomske variacije kot tudi za žilno patologijo vej zgornje mezenterične arterije so lahko že v samem razvoju sprednjih splanhničnih arterij in njihove oskrbe. Poznavanje žilne anatomije in anatomskih variacij je pomembno in dragoceno za radiologe kot tudi kirurge. AbstractSuperior mesenteric artery, the second ventral branch of the abdominal aorta, supplies the distal duodenum, the small intestine, and the large intestine to the mid transverse colon. Superior mesenteric artery branches include the inferior anterior and inferior posterior pancreaticoduodenal arteries, middle colic artery, right colic artery, ileocolic artery, jejunal and ileal branches. The vascular anatomy of superior mesenteric branches is frequently variant. The explanation of variant vascular anatomy of branches and pathological consequences of diseases which impact the mesenteric vasculature might be due to the changes that appear in the development of ventral splanchnic arteries and their blood supply. Knowledge of mesenterical variations is valuable to radiologists and surgeons.

Purpose: The infant mortality rate (IMR) is among the most important indicators of a nation's health and well–being. In Slovenia, over the last 55 years, infant mortality (IM) has decreased rapidly and vaccinations are undoubtedly the major contributing factor. In Slovenia, infant immunization is initiated at the age of 3 months. The vaccination schedule includes several doses of combined vaccines that should be administered according to the yearly program on immunization. Methods: In this retrospective study, 279 infants were divided into subgroups in accordance with the vaccination program. The structure of the study design used the data of the National Institute of Public Health of Slovenia regarding the causes of IM for the years 2007–2011 Results: Direct and indirect leading causes of death were infant respiratory distress syndrome (n = 22), extreme immaturity (n = 19), necrotizing enterocolitis (n = 18), extremely low birth weight (n = 14), and hypoplastic left heart syndrome (n = 14). The maximum IMR (85.30%) occurred before the recommended vaccination period. During the prescribed program of vaccination, the IMR was 14.70%. Conclusion: Genetic alterations, congenital abnormalities, and preterm birth complications are the major and leading causes of IM. Most instances of IM occur within the first two months of life, before the recommended vaccinations. During the recommended vaccination period, IM is low and causes of death are largely congenital and genetic diseases. The results of the comparative study during the recommended vaccination schedule by the end of the first year of life from 2007 to 2011 substantiates that the vaccines are not a major cause of IM.