Truffle mycelia exhibit a complex interaction pattern with host plants and have been extensively studied over the last years as a source of new bioactive compounds. Fungal enzymes possess a wide use in food industry, confectionaries, textiles and leather industries in order to simplify the processing of raw materials. They are often more stable than enzymes derived from other sources. Tuber maculatum and Tuber aestivum mycelia were tested for enzymes production in Petri dishes solid medium conditions. The results showed that Tuber maculatum produced seven extracellular enzymes (amylase, xylanase, laccase, lipase, peroxidase, cellulase and catalase) while Tuber aestivum produced only three enzymes (amylase, peroxidase and catalase).
Spherical shaped 10-20 nm average size silver nanoparticles were prepared by chemical precipitation method and tested in vitro as antimicrobial agent against Staphylococcus aureus. The particles were characterized via UV-Vis spectrophotometer and TEM photography. 2% (w/v) silver nanoparticles cream was formulated to be used as topical treatment of Staphylococcus aureus infected wound in albino rats. The commercially available antibiotic cream fucidin was used as a standard antibiotic. The infection and treatment stages were followed by morphology, histopathology and immunological analysis. The prepared cream showed high healing effect within 4-6 days with no noticeable morphological change in the skin. In addition silver nanoparticles affect the humoral immunity by increase the serum level of IgG, IgA, IgM as comparing with infected-non treated group. The histopathological examination showed formation of inflammatory cells in infected wounds then significant decrease of the inflammation after treatment with silver nanoparticles cream.
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