The susceptibility of whole and rolled cereal and legume seeds to microbial attack within the rumen of cows was examined by incubating seeds in nylon bags in the rumen for 24,48 and 72 hr. With whole, intact seeds the husked cereal grains were less susceptible than naked grains and legume seeds. Inter-varietal differences were observed with maize, oats and barley. Breaking the cereal grains before incubation considerably increased the losses of dry matter. Digestibility trials with non-lactating cows confirmed that barley, sorghum, wheat and oats were poorly digested when given whole, and considerable variation was recorded between individual cows in their ability to digest whole grains. Rolling the grains before feeding made the greatest improvement in digestibility with sorghum, wheat and barley and the least with oats.

This study attempts to develop an experimental gestational diabetes mellitus (GDM) animal model in female Sprague-Dawley rats. Rats were fed with high fat sucrose diet, impregnated, and induced with Streptozotocin and Nicotinamide on gestational day 0 (D0). Sleeping patterns of the rats were also manipulated to induce stress, a lifestyle factor that contributes to GDM. Rats were tested for glycemic parameters (glucose, C-peptide, and insulin), lipid profiles (total cholesterol, triglycerides, HDL, and LDL), genes affecting insulin signaling (IRS-2, AKT-1, and PCK-1), glucose transporters (GLUT-2 and GLUT-4), proinflammatory cytokines (IL-6, TNF-α), and antioxidants (SOD, CAT, and GPX) on D6 and D21. GDM rats showed possible insulin resistance as evidenced by high expression of proinflammatory cytokines, PCK-1 and CRP. Furthermore, low levels of IRS-2 and AKT-1 genes and downregulation of GLUT-4 from the initial to final phases indicate possible defect of insulin signaling. GDM rats also showed an impairment of antioxidant status and a hyperlipidemic state. Additionally, GDM rats exhibited significantly higher body weight and blood glucose and lower plasma insulin level and C-peptide than control. Based on the findings outlined, the current GDM animal model closely replicates the disease state in human and can serve as a reference for future investigations.

Four experiments investigated some of the factors affecting the digestion of whole maize grain in non-lactating cows and yearling steers. With steers, but not cows, maize in diets containing small amounts of hay (14%) and large amounts of whole grain were better digested than those with 37 or 48 % hay. The higher digestibility of maize in steers was associated with a greater time spent ruminating per kg feed and a longer retention time of maize in the gut than in cows. Replacing long roughage with ground and pelleted roughage in diets for steers improved the digestion of whole maize but not as well as reducing the hay to 14% of the diet. Finely grinding the maize produced a large increase in digestibility of the feed in cows and steers and both digested it to the same extent. It was concluded that the motility of the reticulo-rumen during eating and ruminating largely determines the extent of the digestion of whole maize grain.

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