We studied peripheral blood and apheresis samples from 39 consecutive normal donors who were parents or siblings of patients who received matched or mismatched bone marrow transplants using a combination of rhG-CSF-mobilized peripheral blood stem cells (PBSCs) and bone marrow (BM). BM was harvested from donors 1-7 days before starting rhG-CSF treatment: 12 micrograms/kg/day rhG-CSF was administered by continuous s.c. infusion for 4-7 days. Peripheral blood progenitor cells were harvested by leukapheresis using an automated continuous-flow blood cell separator, beginning on day 4 of rhG/CSF, for 1-4 consecutive days. Peak peripheral blood CD34+ cell and CFU-GM levels were reached simultaneously on day 5 or 6 of rhG-CSF administration. Median peak levels were 1.65% for CD34+ cells (range 0.34%-4.7%) and 142 CFU-GM/10(5) plated cells (range 16-700). The greatest numbers of CD34+ cells and CFU-GM, expressed per liter of blood volume processed, were harvested during the second and third leukapheresis: CD34+ cells 37.77 +/- 25.48 x 10(6) and CFU-GM 3.32 +/- 2.51 x 10(6) during the second leukapheresis, and CD34+ cells 37.01 +/- 16.33 x 10(6) and CFU-GM 3.82 +/- 4.36 x 10(6) during the third. The number of CD34+ cells and CFU-GM did not correlate with the sex, age, or body weight of the donors. This study indicates that this protocol for administration of rhG-CSF mobilizes large numbers of hematopoietic progenitor cells into the peripheral blood and that bone marrow harvesting before G-CSF administration does not impair stem cell mobilization.
This paper is part of the design strategies of reassembly and reuse of buildings according to the transition to circular economy. Specifically, the paper addresses the issue of architectural envelopes made of drywall and their executive design according to a Life Cycle Thinking (LCT) approach, based on a case study of a temporary wooden housing unit for which there are several scenarios of use and end of life once the first cycle of use of the components of the envelope has come to an end. In particular, the paper, based on the collection of environmental profiles of products through Environmental Product Declarations (EPD), wants to define a balance between the environmental impacts incorporated in the used materials and the benefits related to the adoption of dry construction techniques.
Within the framework of the research and innovation strategy RIS3 “Sustainable Living” (POR-FSE, funded by the Veneto Region), for the improvement of the resilience and adaptation capacity of the Veneto territory to environmental crises and emergencies, the subject of the contribution returns the results of the participatory experimentation of the project H.E.L.P. Veneto ‘ High-efficiency Emergency Living Prototypes Veneto - Sustainable adaptive residences for temporary stay in environmental emergencies. The research concerns the design of a minimum flexible emergency living module, replicable on a large scale, multifunctional, sustainable, powered by off-grid systems and integrated into the built environment. The housing unit uses timber, a material linked to the local building tradition, whose prefabricated modular reversibility follows principles of circular reuse. Moreover, the constructive adaptability of the interior spaces is reflected in a “liquid space” capable of transforming itself according to the needs of the occupants. The paper introduces a form of participatory design of the emergency housing module, based on the engagement of small and large companies, related to different segments of the construction market, a leading sector in the economy of Veneto. The participatory approach borrows from Architectural Technology the tools needed to understand the characteristics of the settlement system, the potential of the project and the value of scientific stakeholder engagement in the process. Using the Soft System Methodology, direct investigation protocols have been constructed relating to the performance of the living unit. Using Strategic Options Development and Analysis (SODA), the results of the experimented survey (large-scale questionnaires) were decoded, interpreted and systematised. The processing of the answers allowed the stakeholders to validate the potential of the proposed module and, at the same time, to be informed about its characteristics. The innovation of the method lies precisely in the modelling phase, which makes it possible to integrate the results of the hard and soft data analyses and to make it clear how participation plays an essential role in the process of designing and validating the proposed module. Nell’ambito della strategia di ricerca e innovazione RIS3 “Sustainable Living” (POR-FSE, finanziato dalla regione Veneto), per il miglioramento della capacità di resistenza e di adattamento del territorio veneto a crisi ed emergenze ambientali, l’oggetto del contributo restituisce gli esiti della sperimentazione partecipata del progetto H.E.L.P. Veneto High efficiency Emergency Living Proto- types Veneto – Residenze adattive sostenibili per la permanenza temporanea in regime di emergenza ambientale. La sperimentazione riguarda la progettazione di un modulo minimo abitativo di emergenza flessibile, repli- cabile a larga scala, polifunzionale, sostenibile con impianti a funzionamento off-grid e integrato nell’ambiente costruito. L’unità abitativa utilizza il legno, materiale legato alla tradizione costruttiva locale, la cui reversibilità modulare prefabbricata segue principi di riuso circolare. Inoltre, l’adattabilità costruttiva degli ambienti interni si riflette in uno “spazio liquido” capace di trasformarsi in base alle esigenze dell’abitare. Il contributo propone una forma di progettazione partecipata del modulo abitativo emergenziale, basata sull’engagement delle realtà aziendali di piccole e grandi dimensioni, relative ai diversi segmenti di mercato dell’edilizia, settore trainante della regione Veneto. L’approccio partecipativo mutua dalla Tecnologia dell’Architettura gli strumenti di conoscenza atti alla comprensione delle caratteristiche del sistema insediativo, delle potenzialità del progetto e del valore dell’engagement scientifico degli stakeholder nel processo. Utilizzando la Soft System Methodology sono stati costruiti dei protocolli di indagine diretta che combinano la conoscenza prestazionale dei processi insediativi nell’unità ambientale. Mediante la Strategic Options Development and Analysis (SODA) sono stati decodificati, interpretati e sistematizzati i risultati della survey sperimentata (questionari ad ampia scala). L’elaborazione delle risposte ha fatto sì che il sapere esperto degli stakeholder validasse le potenzialità del modulo proposto informandosi, al contempo, sulle caratteristiche dello stesso. L’innovazione del metodo risiede proprio nella fase di modellazione, la quale permette di integrare i risultati delle analisi dei dati hard e quelle dei dati soft, e di rendere chiaro come la partecipazione svolga un ruolo essenziale nel processo di animazione e validazione del modulo proposto.
Although autistic disorder is rare, we identified three children with this syndrome in a sample of 286 children with congenital HIV infection. The prevalence of autistic disorder was thus greater than expected from epidemiological data. The present article describes the clinical manifestations and course of development of the three children. Etiologic heterogeneity in autism is assumed by most investigators because of the occurrence of autistic disorder in persons with a variety of other disorders (e. g. viral or genetic). We hypothesize that there is a complex relation between congenital HIV infection and austistic disorder and suggest the need for systematic investigations of larger series of HIV positive children.
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