Background The current outbreak of the COVID-19 pandemic has distorted the physical, mental, and psychological condition of frontline healthcare providers in health facilities. This study aims to investigate the prevalence, and risk factors of depression, anxiety, and stress among nurses working in a COVID-19 referral hospital in Indonesia. Methods A cross-sectional study was conducted among 491 nurses, aged between 31, and 56 years, using a self-administered questionnaire. The Depression Anxiety Stress Scale (DASS21), and demographic questions were used to screen the presence of psychological problems, and their associated factors. Results The prevalence of moderate to extremely severe depression, anxiety and stress was 8.5 %, 20.6 and 6.3 %, respectively. Regression analysis showed that anxiety was significantly higher among nurses working in non-COVID wards (p = .01), those who experienced social rejection (p < .05), and those who frequently watched television (p < .05). Those who had temporary contracts were more stressed (p < .05), and those who faced financial hardship during the COVID-19 pandemic significantly experienced depression, anxiety, and stress at 10.5 %, 23.5 %, and 8.1 % respectively(p < .05). Conclusions While the prevalence of mental distress in this hospital is low, it exists, and hospital management should consider training for all nurses, public sensitization on COVID-19, and provision of financial subsidies for frontline workers, in order to manage the risk factors.
The growth hormone (GH) is the main regulator of postnatal growth and metabolism in mammals. The action of GH on target cells depends on the growth hormone receptor (GHR). This is mediated through induced transcription of other genes. GHR gene is one of the candidate genes employed in selection strategy using DNA markers (marker assisted selection). This study was designed to identify the novel single nucleotide polymorphisms (SNPs) in exon 8 and intron 8 of GHR gene that may affect production traits in Bali cattle. A SNP was identified by the direct sequencing technique. Genotypes of the SNPs were identified using PCR-RFLP. The SNP was located in intron 8 of the GHR gene and was caused by an A/G transition. It was identified using the HpyCH4III restriction enzyme. Polymorphism of GHR/HpyCH4III has a significant influence on weaning weight and average daily gain, but not on birth weight of Bali cattle.
ABSTRAKInsulin-like growth factor-1 (IGF-1) adalah salah satu kandidat gen dalam strategi seleksi menggunakan marka DNA (marker assisted selection). Strategi kandidat gen merupakan teknik biologi molekuler untuk mengidentifikasi lokus sifat kuantitatif secara langsung, dengan asumsi bahwa variasi genetik kandidat gen ini berasosiasi dengan sifat kuantitatif. Penelitian ini dirancang untuk mengidentifikasi adanya mutasi baru pada exon 4 yang dapat menimbulkan polimorfisme gen IGF-1 yang mempengaruhi sifat produksi pada sapi bali. Pencarian single nucleotide polymorphism (SNPs discovery) dilakukan menggunakan metode "direct sequencing", sedangkan variasi genetik kandidat gen ini diidentifikasi menggunakan teknik "polymerase chain reaction-restriction fragment length polymorphism" (PCR-RFLP). Hasil penelitian ini menunjukkan bahwa pada exon 4 gen IGF-1 ditemukan SNPs baru, yaitu mutasi transisi T/C, yang kemudian dapat identifikasi menggunakan enzim restriksi Rsa1. Polimorfisme genotipe IGF-1/Rsa1 memiliki pengaruh yang signifikan terhadap bobot lahir, bobot sapih, dan rata-rata pertambahan bobot badan harian sapi bali. Genotipe CC memiliki angka bobot lahir, bobot sapih, dan rata-rata pertambahan bobot badan harian berturut-turut: 15,64±1,83; 83,15±9,00; dan 0,439±0,07 yang lebih tinggi dibandingkan genotipe TT dan CT. Genotipe IGF-1/ Rsa1 dapat digunakan sebagai penciri genetik untuk seleksi bobot lahir, bobot sapih, dan pertambahan bobot badan harian.Kata kunci: gen, mutasi, restriksi, polimorfisme, marka, seleksi ABSTRACT Insulin-like growth factor-1 (IGF-1) is one of the gene candidates that can be used in selection strategy by using DNA markers (marker assisted selection). Gene candidate strategy is a molecular biology techniques to identify quantitative trait loci directly, with the assumption that genetic variation associated to quantitative trait variation. This study was designed to identify any new mutations in exon 4 that can cause the IGF-1 gene polymorphism and then affect the production traits on Bali cattle. Single nucleotide polymorphism (SNP) discovery was conducted by using the direct sequencing technique. Genetic variation of the genes candidate was identified by using PCR-RFLP technique. The results of this study indicate the presence of a new SNP in exon 4 of IGF-1 gene caused by the T/C transition, which can be identified using Rsa1 restriction enzyme. Genotypic polymorphism of IGF-1/Rsa1 has a significant influence on birth weight, weaning weight and average daily gain of Bali cattle. CC genotype had a birth weight rate, weaning weight and average daily gain of: 15.64±1.83; 83.15±9.00, and 0.439±0.07 respectively, higher than the TT and CT genotype. IGF-1/Rsa1 can be used as a genetic marker for selection of birth weight, weaning weight, and daily body weight gain.
The World Health Organization in 2009 recorded that more than a million people died due to accidents, and about 2 million people had physical accidents, namely fractures. The main challenge for clinicians today is finding an effective and inexpensive method of fracture healing. In this case, a pulsed electromagnetic field (PEMF) can be used, which is a non-invasive technique that is inductive and generates a magnetic field due to changes in the electric field. So this research aims to create a simulation design of PEMF therapy device for fracture healing. The stages of this research method are to make Helmholtz coil modelling of PEMF therapy device using Comsol Multiphysics with variations in the number of turns, the distance between two coils, voltage or current, and coil diameter, to obtain the correct magnetic field value to accelerate fracture healing. The result of this research is the successful design of a PEMF device that produces a magnetic field value B is 1.5 mT on the number of turns N is 150, the distance between two coils r is 2.5 cm, the current value I is 0.5 A, and coil diameter D is 5 cm.
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