Abstract:With the aid of the antibodies raised against synthetic peptides corresponding to the sequences of human dystrophin, 440-489 and 3495-3544, respectively, dystrophin was isolated from porcine skeletal muscle as a homogeneous protein of about 400 kDa. Main steps of preparation consisted of extraction with weakly alkaline Triton X-100 solution, dimethyl sulfoxide (DMSO) fractionation, gel filtration, cationic chromatography and preparative SDS gel electrophoresis. BrCN treatment of purified dystrophin gave rise to 40 kDa and 10 kDa fractions. Partial sequencing of the 40 kDa fraction revealed that porcine dystrophin was more homologous with human dystrophin than was chicken dystrophin. The 10 kDa fraction reacted strongly with the antibody against the peptide 3495-3544, but not to that against the peptide 440-489, and the sequences of its several parts examined were all identical with those of corresponding parts of human dystrophin, respectively.
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