We generated an aqueous two-phase laminar flow in a microfluidic chip and used the system to isolate leukocyte and erythrocyte cells from whole blood cells. The microfluidic system reduced the effect of gravity in the aqueous two-phase system (ATPS). Poly(ethylene glycol) (PEG) and dextran (Dex) solutions were used as the two phases, and the independent flow rates of the solutions were both 2 microL/min. When hydrophobic and hydrophilic polystyrene beads were introduced into the microfluidic device, the hydrophilic beads moved to the Dex layer and the hydrophobic beads to the interface between the two phases. In the case of living cells, Jurkat cells and erythrocytes moved more efficiently to the PEG and Dex layers, respectively, than they move in a conventional ATPS. When whole blood cells were inserted into the microfluidic chip, leukocytes could be separated from erythrocytes because erythrocytes moved to the Dex layer while leukocytes remained outside of this layer in the microfluidic system. The reported microfluidic chip for the whole blood cell separation can effectively be integrated into a Micro Total Analysis System designed for cell-based clinical, forensic, and environmental analyses.
The single step‐up pressure filtration test was developed to determine the pressure dependence of average specific resistance of the cake formed in ultrafiltration of a variety of nano‐colloids over a wide range of pressure drops across the cake. The values of the average specific resistance at extremely low pressures were obtained from only the flux decline data through the use of the distinct time variation of the pressure drop across the cake generated by using the ultrafiltration membrane with a high hydraulic resistance under the low filtration pressure in the first step of filtration. The values at higher pressures were obtained from the time variation of the filtration rate induced by a stepwise increase in the pressure. The correlations between the average specific cake resistance and the pressure drop across the cake were evaluated using only the flux decline data for a variety of different proteins and nanoparticles. © 2013 American Institute of Chemical Engineers AIChE J, 60: 289–299, 2014
In the last decade, there has been increasing pressure on academic laboratories to produce practical results. The last 10 years also have seen a growing interest in knowledge management, a management discipline believed to enhance organizations' innovative capability by the sharing and creation of knowledge. While most knowledge management cases refer to the business setting, we believe that the introduction of these practices can also enhance knowledge creation and knowledge sharing within and among research units. This paper focuses on a pilot study being conducted at a Japanese public graduate university -JAIST -under a Center of Excellence (COE) program that was established to bring the performance of research laboratories up to a world class level in productivity by applying the theories and tools of knowledge science. This study is a cooperative effort between the School of Knowledge Science, doing research on knowledge management and systems, and two research laboratories in the School of Materials Science, doing basic and applied research on materials science. The goal of this project is to enhance materials science students' capabilities so that they become successful creators of new scientific knowledge. A group of seven graduate research students volunteered for the study. As one of the first steps, we introduced a formal and periodic written reporting system that motivates students to think strategically about their experiments, helps them to improve their communications skills, and enables students to self-evaluate their skills and supervisors to evaluate the students' skills as well as monitor their progress and developments in a formalized way. Since the project is relatively new, these preliminary results are associated with a generalized awareness and participation of the students in the project. However, we are expecting to obtain more concrete results, that is, quantifiable improvements in scientific production, in the near future.
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