Although
it has been demonstrated that membrane proteins (MPs)
require lipids to ensure their structural and functional integrity,
details on how lipid–MP interactions regulate MPs are still
unclear. Recently, we developed a concise method for quantitatively
evaluating lipid–MP interactions and applied it to bacteriorhodopsin
(bR), a halobacterial MP that forms trimers and acts as a light-driven
proton pump. Consequently, we found that the halobacterial glycolipid,
S-TGA-1, has the highest affinity for bR, among other lipids. In this
study, we examined the effects of S-TGA-1 on bR via visible circular
dichroism spectroscopy, flash photolysis, and proton influx measurement.
The results showed that S-TGA-1 efficiently promotes trimer formation,
photocycle, and proton pumping in bR. Our data also suggested that
the bR photocycle is restored as a consequence of the trimerization
induced by the lipid. This study demonstrates clearly that lipids
specifically interacting with MPs can have significant impacts on
MP structure and/or function. The methodology adopted in our studies
can be applied to other MPs and will help elucidate the physiological
functions of lipids in terms of lipid–MP interactions, thus
accelerating “lipid chemical biology” studies.
Membrane proteins embedded in the membrane undergo changes in their actions under the influence of membrane lipids. Here, we present a novel type of lipid action on the potassium channel KcsA from Streptomyces lividans. Cardiolipin is present in various cellular membranes, including the host membrane of KcsA. Although the M0 domain, a non-transmembrane helix, is known to sense anionic lipids in the inner leaflet, we found that divalent anionic cardiolipin in the outer leaflet of the membrane interacts with positively charged residues, Arg64 and Arg89, on the extracellular side of the transmembrane domain. This binding propagates its action across the membrane toward the intracellular region of KcsA, thus, opening the inner gate. Such a long-range allosteric effect has not been found for channel-lipid interactions.
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