Female reproductive success (seed set) of a spring ephemeral plant, Corydalis ambigua Cham. et schlecht (Papaveraceae) was investigated in relation to inflorescence size and foraging behavior (frequency and duration of visitations) by pollinators (namely, overwintered queens of Bombus hypocrita sapporensis) by detailed daily observations of a natural population. Pollination experiments indicated that C. ambigua is self-incompatible and that seed set was significantly affected by the behavior of the pollinating queens. Plants with larger inflorescences were visited more often than those with fewer flowers. Fecundity also increased with increasing size of inflorescences. Visitation time (duration of foraging) rather than the frequency of visitations (number of visits) was critical for higher fecundity. Seed production was strongly enhanced by a few long visits (of more than 60 s), and seemed to be independent of large numbers of short visits (of less than 60 s). Hence, plants with larger inflorescences, which provide a conspicuous signal to pollinators and offer greater rewards in terms of nectar, received longer visits by B. hypocrita sapporensis queens and those plants exhibited higher fecundity.
1. The behaviour of nectar-collecting Bombus hypocritu sapporensis Cockerell queens was observed on a population of a spring ephemeral plant Corydulis umbiguu Cham. et Schlecht.2. Daily patterns of activity and behaviour changed with the progress of flowering. Activity peaked shortly before sunset early in the flowering season but approximately at noon towards the end of flowering. In the peak flowering period the queens tended to visit nearby plants and to change direction often, whereas early or late in the flowering period they flew further between visits and were less likely to change direction.3. Each plant was visited 0 to 24 times (mean 9.4 +SD 5.2) by the queens during the whole flowering season.4. The queens collected nectar, rarely through the front of the flowers but mostly through the spurs perforated by themselves or predecessors. At the beginning of the flowering season the illegitimate foragers often visited the front of the flowers before moving to the spurs; later, most queens quickly learned to land directly on the spurs. 5. Even the 59.7% of plants that were visited only by illegitimate foragers set seeds. Close observation confirmed that the illegitimate foragers opened the inner petals enclosing anthers and stigma frequently when visiting the front of the flowers before robbing, or occasionally when walking about on the flowers or collecting nectar through the perforated spurs.
In alpine ecosystems, microscale variation in snowmelt timing often causes different flowering phenology of the same plant species and seasonal changes in pollinator activity. We compared the variations in insect visitation, pollen dispersal, mating patterns, and sexual reproduction of Rhododendron aureum early and late in the flowering season using five microsatellites. Insects visiting the flowers were rare early in the flowering season (mid-June), when major pollinators were bumblebee queens and flies. In contrast, frequent visitations by bumblebee workers were observed late in the season (late July). Two-generation analysis of pollen pool structure demonstrated that quality of pollen-mediated gene flow was more diverse late in the season in parallel with the high pollinator activity. The effective number of pollen donors per fruit (N(ep)) increased late in the season (N(ep) = 2.2-2.7 early, 3.4-4.4 late). However, both the outcrossing rate (t(m)) and seed-set ratio per fruit were smaller late in the season (t(m) = 0.89 and 0.71, seed-set ratio = 0.52 and 0.18, early and late in the season, respectively). In addition, biparental inbreeding occurred only late in the season. We conclude that R. aureum shows contrasting patterns of pollen movement and seed production between early and late season: in early season, seed production can be high but genetically less diverse and, during late season, be reduced, possibly due to higher inbreeding and inbreeding depression, but have greater genetic diversity. Thus, more pollinator activity does not always mean more pollen movement.
We investigated the effects of forest fragmentation on reproduction of a common understory perennial, Trillium camschatcense, in Hokkaido, Japan. We measured seed production of 12 (1998) and 14 (1999) populations, and we explored the relationships of seed production to the number of flowering plants ( population size) and to the surrounding landscape condition ( landscape type). We discriminated between two landscape types, isolated and continuous, to describe the quantity and distribution of forests around T. camschatcense populations. Population size may be correlated with attractiveness to pollinators and the availability of compatible mates, whereas landscape condition can affect the abundance of pollinators. The number of seeds per flower was related to population size in 1999, but not in 1998. In both years, small populations of <50 flowering plants produced few seeds. Although the effect of landscape type was not significant, continuous populations with abundant forest series in their neighborhood tended to produce a higher number of seeds than isolated populations located in small, isolated forest. We attribute the decrease in seed production to pollen limitation because the stigmatic pollen load had a positive relationship to seed production and addition of pollen by hand increased seed production. Variation in population structure ( four stage classes) among populations was better explained by population size than number of seeds produced, suggesting the relative importance of population size for the demography of T. camschatcense. Larger populations had a higher proportion of seedlings, possibly because large populations had fewer edge effects, which reduce seedling recruitment and survival. Small populations with 220 flowering plants or less showed almost no seedling recruitment. The data on seed production and population structure suggest that continuous populations with at least 1000 flowering plants may be required to avoid the effects of fragmentation.
Erythronium japonicum (Liliaceae) inhabits deciduous mesic forests of Hokkaido, northern Japan. Myrmecochory of this species was investigated, especially the dispersal frequency, the effect of seed predators and the seed fall pattern. In the quadrat census using marked seeds of E. japonicum, the ant Myrmica kotokui frequently transported the seeds. However, the frequency of seed removal was low and most seeds were dispersed as little as 1 m or less. The spatial distribution of E. japonicum individuals was nearly random and most seedlings were established 5-20 cm away from the fertile plants, indicating that even this small scale of seed dispersal contributes to avoiding crowding of seedlings. Some arthropods, e.g. springtails, spiders and ticks, hindered seed dispersal by devouring elaiosomes and seeds. Although ground beetle species also damaged seeds and elaiosomes, a few of them exhibited seed removal behaviour. E. japonicum dropped their seeds not all at once but bit by bit, taking 3-6 days to drop all seeds. This seed-fall pattern was effective in raising the frequency of seed removal by ants and reducing seed predation by some arthropods.
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