1 This study examined the e ect of diazepam (DZP) on phosphoinositide turnover, which plays an important role in the regulation of salivary secretion, in rat parotid acinar cells. 2 DZP (10 79 M to 10 75 M), a potent agonist of both central-and peripheral-type benzodiazepine receptors, dose-dependently decreased inositol 1,4,5-trisphosphate (IP 3 ) production stimulated by carbachol, a muscarinic receptor agonist, in the cells. 3 DZP produced a maximum inhibitory response at a concentration of 10 75 M, with IP 3 production decreased to 63% of maximal levels. The concentration inducing half maximal inhibition of IP 3 production was approximately 3.5610 78 M. 4 An inhibitory response to DZP was produced by a short-term pretreatment (53 min) of the cells and prevented by antagonist and competing ligand for the central-and peripheral-type benzodiazepine receptors,¯umazenil and PK 11195, respectively. 5 DZP showed a non-competitive inhibition of carbachol-stimulated IP 3 production. It did not directly inhibit the activities of GTP-binding regulatory proteins and phosphatidylinositol 4,5-bisphosphate-speci®c phospholipase C (PLC) in the parotid gland membranes, though choline chloride inhibited PLC activity. 6 DZP (10 75 M) attenuated the increase in the intracellular Ca 2+ concentration ([Ca 2+ ] i ) in the cells following stimulation of the muscarinic and a 1 -adrenoceptors. 7 These results suggest that in the parotid acinar cells, DZP inhibits muscarinic receptor-stimulated IP 3 production through benzodiazepine receptors and that PLC activity which produces IP 3 is inhibited by chloride. The decreases in IP 3 and [Ca 2+ ] i in the cells may be connected with the suppression of salivary secretion induced by DZP.
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