Bidens pilosa L. var. radiata SCHERFF (Tachiawayukisendangusa: Musashino Miyako Bidens Pilosa (MMBP)) has been reported to have antiinflammatory and antiallergic properties in the treatment of experimental diseases. We studied the antiinflammatory and anti-allergic effects of enzymatic digested MMBP (eMMBP), MMBP treated with Cellulosine, using an experimental inflammatory and allergic model. Oral administration of eMMBP suspension in carboxy-methyl-cellulose sodium solution inhibited the production of IgE 10 days after immunization with 2,4-dinitrophenylate (DNP)-ascaris in mice. Oral administration of eMMBP suspension inhibited dye exudation in rat skin induced by passive cutaneous anaphylaxis (PCA) and chemical mediators (histamine and substance P). The effects of eMMBP on the inhibition of histamine release from mast cells induced by compound 48/80 or antigenantibody reaction was stronger than that of the hot-water extract (HWex). Caffeic acid and flavonoids (hyperin and isoquercitrin) in eMMBP are increased more than those in HWex of MMBP, as estimated using HPLC. These results suggest that eMMBP has antiinflammatory and antiallergic activity, and that enzymatic digestion enhances its antiallergic activity by the inhibition of histamine release from the mast cells due mainly to an increase in caffeic acid and flavonoids. The enzymatic digestion of MMBP might be useful in enhancing its antiinflammatory and antiallergic activities.
The suspension and the boiling water extract of dried powder from the aerial parts of Bidens pilosa L. var. radiata SCHERFF (Tachiawayukisendangusa: MMBP) on the Japanese island of Miyako have antiinflammatory and antiallergic properties in experimental diseases. Oral administration of MMBP suspension in carboxy-methyl-cellulose sodium solution inhibited the production of IgE 10 days after immunization with DNP-ascaris in mice. The extract inhibited histamine release from rat peritoneal mast cells induced by compound 48/80 or antigen-antibody reaction. Oral administration of the suspension inhibited dye exudation in rat skin induced by passive cutaneous anaphylaxis. Oral administration of the suspension inhibited dye exudation in rat skin induced by chemical mediators (histamine, substance P, and serotonin). These findings suggest that MMBP may be clinically useful in the prevention of type I allergic disease.
Bidens pilosa L. var. radiata Scherff: (BP) is a plant used as a traditional folk medicine. BP, cultivated with only green manure on Miyako Island, Okinawa prefecture, was processed to powder and is referred to as MMBP. We have reported that MMBP has antioxidant, anti-inflammatory, and anti-allergy properties. In this study, we investigated the effects of MMBP on several experimental gastric lesions induced by HCl/EtOH, a non-steroidal anti-inflammatory drug, or cold-restraint stress, comparing these results with those of rutin or anti-ulcerogenic drugs (cimetidine or sucralfate) based on the lesion index and hemorrhage from the gastric lesions. Orally administered MMBP prevented the progression of the gastric lesions. Moreover, treatment with MMBP, rutin, or sucralfate, which had potent antioxidative activity, inhibited increases in the levels of thiobarbituric acid reactive substances (TBARS) in the gastric mucosal lesions. The inhibition of the gastric mucosal TBARS content by MMBP may have been due to the antioxidant effects of MMBP. These results indicate that MMBP prevents the progression of acute gastric mucosal lesions, possibly by suppressing oxidative stress in the gastric mucosa.
The medical mechanism against type I allergies is to block the release or production of chemical mediators from mast cells or to block the H(1)-receptor signaling. We previously reported that the anti-allergic action of the dry powder from Bidens pilosa L. var. radiata SCHERFF treated with the enzyme cellulosine (eMMBP) was dependent on the inhibition of histamine release from mast cells. Here, we investigate that the effect of fractions in eMMBP on the histamine-induced contraction in guinea pig ileum and on the release of histamine in rat peritoneal mast cells. The histamine-induced contraction in guinea pig ileum is dose-dependently inhibited by ketotifen, an antagonist of H(1)-receptor. Fractions contained caffeic acid, caffeoylquinic acid and fractions contained flavonoids such as hyperin and isoquercitrin in eMMBP inhibit histamine release from mast cells, but only flavonoids such as hyperin, isoquercitrin and rutin suppress the histamine-induced contraction in guinea pig ileum. Moreover, the histamine-induced contraction was not affected by caffeic acid, however, such contraction was significantly inhibited by rutin. These results suggest that the primary antagonists of H(1)- receptor are different from the components in eMMBP that inhibit histamine release, and that these components participate in the anti-allergic activity of eMMBP.
Highly thermo- and photostable, near-infrared-absorbing heptamethine cyanine dyes were achieved with the use of fluorine-containing components. In particular, one prepared heptamethine cyanine dye, bearing a tetrakis(pentafluorophenyl)borate as a counter anion and an N-ethyl-2,2,2-trifluoroacetamido group at the meso position, showed not only a high decomposition temperature (Tdt ), but also very high photostability toward white LED irradiation.
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