SummaryTwo kinds of high-speed liquid chromatographic methods using either ultraviolet or a fluorometric detector (HSLC-UV and HSLC fluorometric methods) are described for the determination of ubiquinones (UQ). HSLC-UV method: Serum or liver was saponified with methanolic alkaline solution containing pyrogallol as an antioxidant and 2, 3, 6 trimethyl-5-nonaprenyl-l, 4-benzoquinone as an internal standard and then extracted with n-hexane. The extract was evaporated and then dissolved in dioxane. The resulting solution was applied to a HSLC system using a Permaphase ODS column, ethanol-water (80:20) as a mobile phase and a high sensitivity UV detector (275nm). HSLC-fluorometric metho: Serum was deproteinized with ethanol and then extracted with n-hexane. The resulting extract was reacted with alkaline ethylcyanoacetate (ECA) reagent to give fluorescence. The reacted solution was applied to a HSLC system using a Permaphase ODS column, ethanol-water (75: 25) as a mobile phase and a spectrofluorometer (Ex. 430nm, Em. 530nm). Neither of the proposed HSLC methods were affected by other fat-soluble substances and seemed to be more simple, sensitive and specific than other conventional determination methods.It is well known that ubiquinones (UQ) play an important role in the mitochondrial electron transport chain of respiration . These compounds are also used for the therapy of human diseases. In order to investigate the role of UQ in animals and human beings, it is necessary to establish a simple and precise method for the determination of these compounds in biological materials .

No abstract

No abstract

No abstract

No abstract