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A potentiometric sensor is reported for the mercury(II) detection, which uses substituted thiourea-functionalized nanoporous silica (FTU-LUS-1) as the sensitive material. Substituted thiourea (FTU) and FTU-LUS-1 were first prepared and then characterized . The electrode exhibited a detection limit of 7.0 × 10 -8 mol dm -3 . Moreover, the selectivity coefficient, response time, performance, sensitivity and stability of the modified electrode were investigated. The electrode presented a response time of about 35 s, a high performance and sensitivity in a wide range of cation activities as well as good long term stability (more than 9 months). The method was satisfactory and could also be used to monitor the mercury(II) ion concentration in waste water and fish samples.