Extended-spectrum beta-lactamases (ESBLs) and metallo beta-lactamases (MBLs) are compromising the chemotherapeutic use of cephalosporins and carbapenems respectively. This study investigated the burden of ESBLs and MBLs co-production among multi-drug resistant (MDR) Gram-negative bacteria collected from two tertiary hospitals in Oyo State. A total of 240 non-duplicated clinical isolates of Escherichia coli, Klebsiella spp. and Pseudomonas spp. were collected from the Microbiology units of two tertiary hospitals in Oyo State and their identities authenticated using standard identification techniques. Antimicrobial susceptibility testing was carried out by disc-diffusion method and isolates exhibiting resistance to ≥3 classes of antibiotics selected as MDR strains. ESBL and MBL production was detected by double-disc synergy test (DDST) and combined-disc-diffusion test (CDDT) respectively. Selected beta-lactamase genes were detected by PCR, amplicons sent out for sequencing and phylogenetic tree of the sequences constructed using Mega X software. MDR was exhibited by 43.8% of the isolates. ESBLs and MBLs were produced by 32.4% and 7.6% of the MDR isolates respectively. Co-production of ESBL and MBL was observed in 6.7% of the MDR isolates. BlaCTX-M-15 (67.7%), blaTEM-1 (55.9%), blaSHV-1 (47.1%), co-existing blaTEM + blaSHV, blaTEM + blaCTX-M, blaCTX-M + blaSHV (each in 5.9%) and blaCTX-M +blaTEM + blaSHV (26.5%) were detected among the ESBL-producers. MBL genes were not detected among the MBL-producers. Only blaTEM-1 sequences showed two different claudes on the phylogenetic tree. The occurrence of MDR isolates co-harbouring different classes of beta-lactamse genes observed in this study is of public health concern and hence, requires stricter control of antibiotic use.
Diseases and infections which are naturally transmitted between animals and humans are of major concern worldwide. Geckos (Hemidactylus frenatus) are known to be potential reservoirs of many zoonotic enteropathogens. This study was designed to isolate, identify, and evaluate the antibiotic susceptibility pattern of Enterobacteriaceae from Geckos. Using standard microbiological procedures, bacteria were isolated from 138 intestinal samples of Hemidactylus frenatus collected from different sampling sites. A total of 20 bacterial species of 9 different genera were identified using automated Colorimetry VITEK 2 system. The percentage occurrences were Enterobacter aerogenes (35%), Proteus mirabilis (15%), Salmonella ser paratyphi B (10%), Serratia fonticola (10%), Enterobacter kobei (10%), Raoultella ornithinolytica (5%), Sphingomonas paucimobilis (5%), Acinetobacter baumannii, (5%) and Burkholderia cepacia (5%). Results obtained from the antibiotic susceptibility pattern according to CLSI guidelines revealed that all the 20 bacterial species have varying rate of resistance with 20 (100%) showing resistance to Ciprofloxacin (CPX), 20 (100%) Pefloxacin (PEF), 19 (95%), Augmentin (AU), 11 (55%) Cotrimoxazole (CXT), 10 (50%) Streptomycin (S), 9 (45%) Chloramphenicol (CH), 6 (30%) Gentamycin (CN), 3 (15%) Ofloxacin (OFX). This study revealed that Enterobacteriaceae in the intestine of Geckos are multidrug resistant and are potentially harmful when in contact directly or indirectly with humans. It becomes important to educate people on the importance of personal hygiene in order to eradicate Geckos from our environment.
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