Herein, we report a dual dye competitive
screening method for the
identification of five boronic acid functionalized synthetic lectins
(SLs) that are selective for prostate-associated targets with the
goal of detecting and staging prostate cancer. This method uses differently
labeled normal (RWEP-1) and diseased (PC3) cell membrane extracts
in a competitive binding assay to identify SLs that bind either the
cancerous or normal extracts but not both. Subsequent studies examined
the efficacy of these new SL hits in an array format to discriminate
six prostate cell lines. The SL array was able to (a) classify the
prostate cell lines with 83% accuracy, (b) discriminate the same cell
lines based on their metastatic potential (noncancerous/healthy, cancerous/lowly
metastatic, and cancerous/metastatic) with 96% classification accuracy,
and (c) exhibit enhanced selectivity for prostate-derived versus colon-derived
cell lines. Further analysis delineated the contribution from each
SL in these studies, providing a focused SL array having potential
utility as a cancer diagnostic.
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