Derivative spectrophotometric (first, second, third and fourth derivative) were developed for the determination of Sulphamethoxazole)SMX) and trimethoprim (TMP)antibiotic binary mixture by applying zero-crossing technique for pure synthetic mixture while simultaneous determination of (SMX) and (TMP) in tabletsusing D 4 at waveleanth 257.8 nm and 251.5 nm for (SMX) and (TMP) the correlation coefficient of calibration curves were 0.9992 and 0.9995. Linearity is maintained within a wide concentration range from 2.00 to 25.00 mg/l, with relative error (-0.11 and 0.60) the LOD (0.360 and 0.382 mg/L) and LOQ and (1.200 and1.275 mg/L) respectively.No interference found between both determined and those of matrices. A good accuracy and precision of simultaneous determination of (SMX), and (TMP) were confirmed by statistical analysis.The percentage recovery of the individual drugs under the established conditions is ranged from 97.23% to 102.13 %. This method was compared with the British pharmacopoeiamethodusing F test. The procedures do not require any separation step.
Derivative spectrophotometric (first, second, third and fourth derivative) were used for the determination of paracetamol)PAR) and hyoscine n-butyl bromide (HYO) the binary mixture by applying zero-crossing technique for pure synthetic mixture. Also simultaneous determination of PAR and HYO in (SPAZMOTEK PLUS) tablets was applied. PAR was determined by using 1 D and 2 D methods at 297.4 and 303.5 nm (zero crossing point of HYO) with linear concentration ranges 2-30 μg.mL-1 , with correlation coefficient r (0.9998, 0.9987), LOD (0.081, 0.250 μg mL-1), relative error (0.68, 0.22) and RSD% (0.107, 0.400), respectively. HYO was determined by using 1 D method at 215.9 nm (zero crossing point of PAR) with concentration range 2-25 μg mL-1 , with correlation coefficient 0.9997 and LOD 0.091μg mL-1 , relative error 1.20 and RSD% 0.342. No interference found between both determined and those of matrices. A good accuracy and precision of simultaneous determination of PAR, and HYO were confirmed by statistical analysis. The percentage recovery of the individual drugs under the established conditions is ranged from 95.07% to 100.93%, the proposed procedures were successfully applied to the determination of these compounds in different ratio by synthetic mixtures and pharmaceutical tablets without requiring any separation step.
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