The study was carried out in the nursery of the Experimental Station of Forestry and Wood Technology Department, Faculty of Agriculture, Alexandria University during the period from 1 st May, 2017 to 31 st March, 2018. It aimed at evaluating the influence of two races of Rhizobium (TAL82 and TAL582) on the overall biomass and the total N content in the leaves of Leucaena leucocephala seedlings. In addition, the study aimed at finding out, if any, the impact of rock-phosphate )RF) amendment on the nodulation and growth efficiency of L. leucocephala seedlings. Seedlings were inoculated with pure cultures of both strains, then fertilized with rockphosphate on 7 th October, 2017 using 4 levels; 0.0, 0.5, 1.0 and 2.0g / kg soil. Both strains (TAL82 and TAL582) developed typical nodules. However, solubility of RF potential was affected by the strain tested as expressed in terms of growth parameters. The strain TAL582 showed, better and faster growth promoting effect than TAL82 in L. Leucocephala seedlings. The fertilization with P induced promoting nodule formation earlier than unamended control. Results showed that fertilization with 1g RF/ kg soil is more effective than the higher tested level (2g RF/ Kg soil). Therefore, it is recommended to inoculate seedlings of L. Leucocephala with Rhizobium particularly TAL582 strain and to add P as a fertilizer at the rate of 1gRF/Kg soil to achieve the best plant growth in significant short time.
Salinity is a devastating environmental stress factor that severely affects plant growth and development. Soil salinity often hinders plant productivity in both natural and agricultural settings. Vesicular Arbuscular mycorrhizal fungal (VAM) symbionts can mediate plant stress responses by enhancing salinity tolerance. Experiments were conducted in a greenhouse at the nursery of the Experimental Station of Forestry and Wood Technology Dept., Faculty of Agriculture, University of Alexandria, Abies region, Alexandria, from June, 2017 to May, 2018 and repeated at the same time in the second season. The obtained results showed that the inoculation with VAM and addition of RP led to enhance the growth significantly, in terms of survival, shoot height, shoot root ratio, root dry weight, shoot dry weight and total dry weight and minerals of the leaves of M. oleifera (N, P and K%) compared with the uninoculated ones. Chlorophyll a of M. oleifera was affected by salinity. Na Cl treatments caused a decrease in chlorophyll a and chlorophyll b content in both seasons. The largest increases in plants nutrient uptake (N, P and K) and decreasing in Na were observed with the VAM+RP treatment. The inoculated seedlings with VAM induced the highest value in Proline content in the first and second seasons compared with the uninoculated ones.The present study concluded that (M. oleifera Lam.) could tolerate salt concentration up to 171.1 mM in the presence of mycorrhiza. It is recommended; however, to inoculate the seedlings with VAM and (1g/kg soil) rock-phosphate application to enhance its growth and mitigate salinity stress.
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