P ESTE des petits ruminants (PPR) is an intense and extremely contagious disease with high mortality in small ruminants resulting inconsiderable socio-economic impact in developing countries. Our study was established during the routine follow up in El-Giza governorate during 2019. A total number of 45 swabs, 40 sera samples and 15 post-mortem epithelial tissues were collected from sheep showing clinical signs suggestive for PPR.PPRVdetection was investigated using serological, pathological and RT-PCRdiagnosis tests.Serologically, PPRV antigens detection were 86%, 93.3%, 66.63% and 73.3% in the nasal, ocular, faecal swabs and different tissues samples, respectively. While, PPRV antibodies detection were 95% in the collected sera samples.Histopathologically, the lung showed severe bronchiectasis and syncytium formation with high accumulation of inflammatory cells. The liver exhibited necrosis with severe portal and central hypertension filled with inflammatory cells.The kidneys revealed severe atrophied glomeruli with destructed renal glomerular capillaries, hemorrhage in the glomeruli and renal tubules with thrombosis of blood vessels. Spleen revealed proliferation of central arterioles with periarteriolar proliferation of connective tissues and hypercellular red pulp with hemorrhage. The mouth commissures suffering from nodules and ulceration which exhibited microscopically multiple vesicles in the epidermis and dissociation of the epidermal and dermal layers filled with inflammatory cells and eosinophils with syncytium formation. A 448 bp band by RT-PCRwas obtained from all nasal and ocular samples, while 2 lung samples were negative for this test.Therefore, the results proved the presence of the disease in El-Giza governorate.
This study aims to employ urine as an easily accessible sample for the detection of bovine viral diarrhea virus (BVDV). Thus, a periodic scan can be applied easily to reach an efficient control of the disease. A total of 86 different samples collected from 30 animals (dams n=23 and calves n=7) were raised in a farm with 1,200 cattle in the El-Fayoum district in Egypt with a case history of abortions were used for this study. All samples were subjected to virological investigation using indirect fluorescent antibody technique (IFAT) and virus neutralization tests (VNT) to detect BVDV. Isolation of the virus was achieved using MDBK cells. The isolates were confirmed using reverse transcriptase polymerase chain reaction. The BVDV was detected in nasal swabs in 63.33% and 70% using IFAT and VNT. In urine samples, the virus was detected with 46.66% and 43.33% by IFAT and VNT, respectively. BVDV was detected in most of the tissues of aborted fetuses in both techniques. Vaginal swabs revealed positive results in 53.85% and 61.54% by IFAT and VNT, respectively. Isolates were confirmed by RT-PCR by successful amplification of 288bp. Statistical analysis revealed good correlation of urine samples and other samples. In conclusion, the BVDV virus can be isolated from urine samples. To the best of our knowledge, this is the first work that provides an overview of urine usability as an alternative sample for BVD virus diagnostics in Egypt. The recommendation for further studies should elucidate on a large scale population.
Bovine herpesvirus 1 (BoHV-1) is known to cause reproductive disorders in Sudanese camels. Egypt imports about 90% of its camels from Sudan, and the rest from Somalia. The BoHV1 is a viral disease of bovines that can be transmitted to camel, sheep, and goat. Due to the absence of anti-camel conjugated with fluorescein isothiocyanate (FITC) in the market, we used protein-A conjugated with FITC which binds to the Fc region of IgG of many animal species. We, therefore, prepared rabbit anti-camel IgG conjugated with FITC and compared it with protein-A conjugated with FITC to the specificity and sensitivity of these compounds in IBR detection from 35 nasal swaps in imported Egyptian dromedary camels. The sensitivity and specificity of the prepared anti-camel IgG FITC and protein-A FITC were compared using Virus Neutralization Test. The labeled protein concentration in the prepared anti-camel conjugate was 2 mg/ml which was considered as an acceptable value. The degree of labeled protein (DOL) was 5.74 cm-1M-1 and optimal DOL usually fell between 2 and 10. The titer of the prepared anti-camel IgG-FITC was 3,125. The prepared anticamel IgG-FITC and protein-A-FITC showed a sensitivity of 93.75 and 90.9%, and a specificity of 71.43% and 62.5%, respectively. Our findings show no significant difference between protein-A conjugated FITC and prepared anti-camel IgG-conjugated FITC in the rapid diagnosis of BoHV-1 in Egyptian dromedary camels. ـــــــــــــــــــــــــــــــــــــــــ This is an open access article under the term of the Creative Commons Attribution 4.0 (CC-BY) International License . To view a copy of this license, visit http://creativecommons.org/licenses/ by/4.0/ J. Appl. Vet. Sci., 5(2 ): 61-66.
Rift Valley Fever (RVF) is an acute infectious zoonotic arthropod-born viral disease, affecting many species of animals and it causes great economic losses in animal wealth and has a zoonotic implication. Eradication of mosquito and vaccination is an important and best method for preventing and controlling the disease. This study was applied for the preparation and evaluation of Rift Valley Fever inactivated vaccine in a lyophilized form that is reconstituted at the time of inoculation using saponin that acts as an adjuvant. The prepared vaccine was proved to be sterile and safe. ED 50 Potency test of the prepared vaccine in mice gave 0.0010 ED 50 /ML (permissible limit less than 0.02/ml). Sheep were vaccinated with 1ml S\C of the prepared lyophilized inactivated RVF vaccine and another group was vaccinated S|C with 1ml of Aluminum hydroxide inactivated RVF vaccine. The immune response of different vaccinated sheep groups was evaluated using SNT and ELISA. The Prepared lyophilized inactivated RVF vaccine gave protective NI at the second-week post-vaccination (2.1 and 0.286 OD) , then reach to peak at the 4 th week (3.28 and 0.343OD) then began to decline till ten months (1.8 and 0.241 OD), while the Aluminum hydroxide inactivated RVF vaccine gave protective NI at the second-week postvaccination (1.7 and 0.277 OD) then reach to its NI peak at the 4 th week (3.13 and 0.312 OD) then began to decline till ten months (1.73 and 0.228 OD). From the obtained results, it was found that the prepared lyophilized inactivated RVF vaccine was safe, potent and gave approximately similar immune response as aluminum hydroxide inactivated RVF vaccine but it is better as it reduces time and effort consuming during vaccine production.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.