Novel biological subtypes and clinically important genetic aberrations (druggable lesions, prognostic factors) have been described in B-other acute lymphoblastic leukemia (ALL) during the last decade; however, due to a lack of studies on unselected cohorts, their population frequency and mutual associations still have to be established. We studied 110 consecutively diagnosed and uniformly treated childhood B-other patients using single nucleotide polymorphism arrays and whole exome/transcriptome sequencing. The frequency of DUX4 -rearranged, BCR-ABL1 -like, ZNF384 -rearranged, ETV6-RUNX1 -like, iAMP21 and MEF2D -rearranged subtypes was 27%, 15%, 5%, 5%, 4%, and 2%, respectively; 43% of cases were not classified into any of these subtypes (B-rest). We found worse early response to treatment in DUX4 -rearranged leukemia and a strong association of ZNF384 -rearranged leukemia with B-myeloid immunophenotype. Of the druggable lesions, JAK/STAT-class and RAS/RAF/MAPK-class aberrations were found in 21% and 43% of patients, respectively; an ABL-class aberration was found in one patient. A recently described negative prognostic factor, IKZF1 plus , was found in 14% of patients and was enriched in (but not exclusive for) BCR-ABL1 -like subtype. PAX5 fusions (including 4 novel), intragenic amplifications and P80R mutations were mutually exclusive and only occurred in the B-rest subset, altogether accounting for 20% of the B-other group. PAX5 P80R was associated with a specific gene expression signature, potentially defining a novel leukemia subtype. Our study shows unbiased European population-based frequencies of novel ALL subtypes, recurrent (cyto)genetic aberrations and their mutual associations. This study also strengthens and widens the current knowledge of B-other ALL and provides an objective basis for optimization of current genetic diagnostics.

In Europe, a broad variety of agricultural landscape types have originated as a result of traditional farming activities and landscape diversity maintenance over centuries. The rapid development of socio-economic activities during the twentieth century caused significant loss of traditional rural landscapes. Traditional/historical European agricultural landscape types (EALs) represent a type of cultural landscape with many specific unique cultural, historical, and biodiversity patterns. Despite their high value, maintenance in practice is lacking. European farmers and landowners need to learn how to implement innovative multifunctional farming techniques within these landscapes. An online interactive educational tool of the ERASMUS+ FEAL project (FEAL: multifunctional Farming for the sustainability of European Agricultural Landscapes) deals with these topics. Case studies from the FEAL project showed the best examples of sustainable agricultural management practices in different types of EALs. The aim of this article was to evaluate case studies within coordination of information on the environment (CORINE) Land Cover (CLC) 2012 classes representing traditional land use forms, nature and landscape protection areas, and ecologically important areas, as well as High Nature Value (HNV) farmland. Results based on 28 case studies from five European countries interpreted the positive external effects of farms on values of EALs. A prevailing number of farms exhibited a coincidence between CLC 2012 classes with traditional land use forms and HNV farmland and protected areas. Regarding land cover classes with traditional land use forms, key words selected by farmers gave importance to recreation and tourism, furthering of biodiversity, direct sale, social farming, renewable energy, and traditional building. The highest frequencies of the key words were achieved in CLC 2012 classes concerning (to some degree) natural and semi-natural ecosystems.

The emergence of cisplatin (CDDP) resistance is the main cause of treatment failure and death in patients with testicular germ cell tumors (TGCT), but its biologic background is poorly understood. To study the molecular basis of CDDP resistance in TGCT we prepared and sequenced CDDP-exposed TGCT cell lines as well as 31 primary patients’ samples. Long-term exposure to CDDP increased the CDDP resistance 10 times in the NCCIT cell line, while no major resistance was achieved in Tera-2. Development of CDDP resistance was accompanied by changes in the cell cycle (increase in G1 and decrease in S-fraction), increased number of acquired mutations, of which 3 were present within ATRX gene, as well as changes in gene expression pattern. Copy number variation analysis showed, apart from obligatory gain of 12p, several other large-scale gains (chr 1, 17, 20, 21) and losses (chr X), with additional more CNVs found in CDDP-resistant cells (e.g., further losses on chr 1, 4, 18, and gain on chr 8). In the patients’ samples, those who developed CDDP resistance and died of TGCT (2/31) showed high numbers of acquired aberrations, both SNPs and CNVs, and harbored mutations in genes potentially relevant to TGCT development (e.g., TRERF1, TFAP2C in one patient, MAP2K1 and NSD1 in another one). Among all primary tumor samples, the most commonly mutated gene was NSD1, affected in 9/31 patients. This gene encoding histone methyl transferase was also downregulated and identified among the 50 most differentially expressed genes in CDDP-resistant NCCIT cell line. Interestingly, 2/31 TGCT patients harbored mutations in the ATRX gene encoding a chromatin modifier that has been shown to have a critical function in sexual differentiation. Our research newly highlights its probable involvement also in testicular tumors. Both findings support the emerging role of altered epigenetic gene regulation in TGCT and CDDP resistance development.

The intention of the article is to demonstrate how data from historical maps might be applied in the process of flood risk assessment in peri-urban zones located in floodplains and be complementary datasets to the national flood maps. The research took place in two industrial parks near the rivers Žitava and Nitra in the town of Vráble (the oldest industrial park in Slovakia) and the city of Nitra (one of the largest industrial parks in Slovakia, which is still under construction concerning the Jaguar Land Rover facility). The historical maps from the latter half of the 18th and 19th centuries and from the 1950s of the 20th century, as well as the field data on floods gained with the GNSSS receiver in 2010 and the Q100 flood line of the national flood maps (2017), were superposed in geographic information systems. The flood map consists of water flow simulation by a mathematical hydrodynamic model which is valid only for the current watercourse. The comparison of historical datasets with current data indicated various transformations and shifts of the riverbanks over the last 250 years. The results proved that the industrial parks were built up on traditionally and extensively used meadows and pastures through which branched rivers flowed in the past. Recent industrial constructions intensified the use of both territories and led to the modifications of riverbeds and shortening of the watercourse length. Consequently, the river flow energy increased, and floods occurred during torrential events in 2010. If historical maps were respected in the creation of the flood maps, the planned construction of industrial parks in floodplains could be limited or forbidden in the spatial planning documentation. This study confirmed that the flood modelling using the Q100 flood lines does not provide sufficient arguments for investment development groups, and flood maps might be supplied with the data derived from historical maps. The proposed methodology represents a simple, low cost, and effective way of identifying possible flood-prone areas and preventing economic losses and other damages.

The knowledge of tree characteristics, especially the shape of standing trees, is important for living tree volume estimation, the computation of a wide range of forest stand features, and the evaluation of stand stability. Nowadays, nondestructive and accurate approaches to data collection in the forest environment are required. Therefore, the implementation of accurate point cloud-based information in the field of forest inventory has become increasingly required. We evaluated the stem curves of the lower part of standing trees (diameters at heights of 0.3 m to 8 m). The experimental data were acquired from three point cloud datasets, which were created through different approaches to three-dimensional (3D) environment modeling (varying in terms of data acquisition and processing time, acquisition costs, and processing complexity): terrestrial laser scanning (TLS), close-range photogrammetry (CRP), and handheld mobile laser scanning (HMLS) with a simultaneous localization and mapping algorithm (SLAM). Diameter estimation errors varied across heights of cross sections and methods. The average root mean squared error (RMSE) of all cross sections for the specific methods was 1.03 cm (TLS), 1.26 cm (HMLS), and 1.90 cm (CRP). TLS and CRP reached the lowest RMSE at a height of 1.3 m, while for HMLS, it was at the height of 8 m. Our findings demonstrated that the accuracy of measurements of the standing tree stem curve was comparable for the usability of all three devices in forestry practices.

Slope failures are financially devastating natural hazards that contribute to land degradation in many areas throughout the world. The adaptation of civic technologies (Google Tango) in a field survey of landslides was examined. Data acquired from different resources and processed using different technologies were merged into a single model to concurrently demonstrate the interoperability and scalability of these data and the model quality. Reference control points were established using a survey‐grade Topcon Hiper SK global navigation satellite system receiver and a Topcon GPT 9003 M total station. An aerial survey was performed in an area of approximately 30,000 m2 using airborne laser scanning (9 points/m2) and aerial photogrammetry using a remotely piloted aircraft system (500 points/m2). The models suffered from data gaps in less visible areas, and micro‐scale landforms reflecting landslide activity were poorly visible. The missing details were supplied using data obtained from close‐range photogrammetry (9,132 m2; 92,300 points/m2) and a Lenovo Phab 2 Pro running Google Tango, which acquired detailed point clouds in near real‐time conditions (1,847 m2; 109,000 points/m2). Scans using the phablet provided point clouds with homogeneously dispersed data gaps, but the spatial accuracy was lower. However, the ergonomics of its field use and its low cost made it competitive with other technologies. The results confirmed that models based on point clouds acquired using different technologies allow the identification and measurement of micro‐scale landforms that may indicate landslide activity.

Wooded grasslands have always played an important role in rural life with changing issues: They are of high importance for questions of biodiversity, soil, and water resources and in preserving agricultural heritage, but their maintenance is labor intensive. Abandoned wooded grasslands undergo succession, and food production alone does not support their survival. They require special attention and at the beginning a well-established subsidy system can help to contribute to their survival. Their sustainable use in the present-day landscapes can only be conceivable in complexity where food production, reintroduction of their cultural values, biodiversity and landscape protection, and ecotourism are playing an important role. This chapter gives an overview on the recent situation of wooded grasslands and their historical development, based on the work done by the Institute for Research on European Agricultural Landscapes (www.​eucalandnetwork.​eu). National pictures, definitions, history (including local names), threats, potentials, cultural values, spatial distributions, subtypes, and available databases have been collected, described, and analyzed. The main results of this survey are as follows: (1) Wooded grasslands are known to the public but mainly to local communities where they occur; (2) Many subtypes of wooded grasslands exist in various European countries; (3) Wooded grasslands underwent tremendous changes during the past centuries and lost their importance for various reasons; (4) There are many local and regional projects focusing on wooded grasslands, often as “lighthouse” projects to valorise cultural achievements

Slámová M., Jančura P., Daniš D.: Methods of historical landscape structures identification and implementation into landscape studies. Ekológia (Bratislava), Vol. 32, No. 3, p. , 267-276, 2013. Valuable historical rural landscapes are found in the sub-mountainous and mountainous regions of the Carpathian Mountains in Slovakia. Authors contributed to the research about historical landscape structures (HLS) by several methods. Method of 'identification and assessment of characteristic landscape' was developed in order to provide maintenance to about HLS and improve application of responsibilities resulting from the European Landscape Convention (Florence, 2000) into practice of landscape planning. We bring a new perspective on landscape's value identification in the field of landscape ecology. The main aim of the paper is identification of HLS as components of land cover structures in the cadastral area of Budina (agrarian terraces) and as micro-relief forms in Nižná Boca (mines). Studied areas represent two different rural landscape types which contain different values related to HLS and they are not especially protected by laws. We evaluate attributes of relief, visual-optometric parameters of landscape, landscape types, land cover structures and types of HLS. Maintenance about HLS in landscape is important for the preservation of unique types of cultural landscapes. Finally, we compare realisations of the visions, suggested in previous landscape studies, which concentrated on development of tourism in the studied areas.Key words: historical structures, land cover, micro-relief forms, landscape types, values, landscape studies. significance and value of historical landscape structures in landscape typesSlovakia has a markedly rural character and according to the 'National strategy of the rural development of the SR for the years 2007-2013' , rural areas cover 86% of the country and 40% of all inhabitants living there (Ministry of Agriculture of the SR, 2012). Valuable historical rural landscapes with historical landscape structures (HLS) are situated mainly in the Carpathian Mountains in the sub-mountainous and mountainous regions in Slovakia (EEA, 2010). We studied relics of traditional agricultural terraces in case of Budiná cadastral area, and in the second case, we studied mediaeval mines in Nižná Boca. The main goal of the paper is identification of HLS in different rural landscape types, assessment of their value and suggestion of recommendations for future management. In both cadastral areas, the value of