Polychlorinated biphenyls (PCBs) represent a large group of recalcitrant environmental pollutants. Up to now, many studies have focused on bioremediation of PCBs by fungal strains; however the mechanisms of adaptation of these strains towards PCBs remain unknown despite their importance in developing effective bioremediation processes. We studied five species, each consisting of two strains isolated either from PCB-polluted or unpolluted substrates (control strains). We investigated their responses to PCB contamination by studying their tolerance to PCBs, their ability to reduce these pollutants and their expression level of Laccase genes. In Thermothelomyces thermophila, Thermothelomyces heterothallica, Thermoascus crustaceus and Fusarium solani, all the studied strains showed a similar tolerance and PCB degradation regardless of their origin. In Schizophyllum commune, while both strains showed similar resistance to PCBs i.e PCBs and their degradation products presented no toxicity for these strains, the rate of PCB degradation of the strain from a PCB-polluted environment was significantly slightly higher. The PCB degradation did not correlate with the expression level of genes encoding Laccases. These results demonstrate that the tolerance and PCB degradation by the fungal strains, which did not involve Laccase activities, required different adaptation systems which seem to be constitutive or rapidly inducible by PCB according to the fungal species.