Motivation: PSORTb has remained the most precise bacterial protein subcellular localization (SCL) predictor since it was first made available in 2003. However, the recall needs to be improved and no accurate SCL predictors yet make predictions for archaea, nor differentiate important localization subcategories, such as proteins targeted to a host cell or bacterial hyperstructures/organelles. Such improvements should preferably be encompassed in a freely available web-based predictor that can also be used as a standalone program.Results: We developed PSORTb version 3.0 with improved recall, higher proteome-scale prediction coverage, and new refined localization subcategories. It is the first SCL predictor specifically geared for all prokaryotes, including archaea and bacteria with atypical membrane/cell wall topologies. It features an improved standalone program, with a new batch results delivery system complementing its web interface. We evaluated the most accurate SCL predictors using 5-fold cross validation plus we performed an independent proteomics analysis, showing that PSORTb 3.0 is the most accurate but can benefit from being complemented by Proteome Analyst predictions.Availability: http://www.psort.org/psortb (download open source software or use the web interface).Contact: psort-mail@sfu.caSupplementary Information: Supplementary data are availableat Bioinformatics online.
Clustering is an important task in mining evolving data streams. Beside the limited memory and one-pass constraints, the nature of evolving data streams implies the following requirements for stream clustering: no assumption on the number of clusters, discovery of clusters with arbitrary shape and ability to handle outliers. While a lot of clustering algorithms for data streams have been proposed, they offer no solution to the combination of these requirements. In this paper, we present DenStream, a new approach for discovering clusters in an evolving data stream. The "dense" micro-cluster (named core-micro-cluster) is introduced to summarize the clusters with arbitrary shape, while the potential core-micro-cluster and outlier micro-cluster structures are proposed to maintain and distinguish the potential clusters and outliers. A novel pruning strategy is designed based on these concepts, which guarantees the precision of the weights of the micro-clusters with limited memory. Our performance study over a number of real and synthetic data sets demonstrates the effectiveness and efficiency of our method.
http://www.psort.org/psortb/supplementaryinfo.html.
Abstract. Both, the number and the size of spatial databases, such as geographic or medical databases, are rapidly growing because of the large amount of data obtained from satellite images, computer tomography or other scientific equipment. Knowledge discovery in databases (KDD) is the process of discovering valid, novel and potentially useful patterns from large databases. Typical tasks for knowledge discovery in spatial databases include clustering, characterization and trend detection. The major difference between knowledge discovery in relational databases and in spatial databases is that attributes of the neighbors of some object of interest may have an influence on the object itself. Therefore, spatial knowledge discovery algorithms heavily depend on the efficient processing of neighborhood relations since the neighbors of many objects have to be investigated in a single run of a typical algorithm. Thus, providing general concepts for neighborhood relations as well as an efficient implementation of these concepts will allow a tight integeration of spatial knowledge discovery algorithms with a spatial database management system. This will speed-up both, the development and the execution of spatial KDD algorithms. For this purpose, we define a small set of database primitives, and we demonstrate that typical spatial KDD algorithms are well supported by the proposed database primitives. By implementing the database primitives on top of a commercial database management system, we show the effectiveness and efficiency of our approach, experimentally as well as analytically. The paper concludes by outlining some interesting issues for future research in the emerging field of knowledge discovery in spatial databases.
Computational prediction of the interaction between drugs and targets is a standing challenge in the field of drug discovery. A number of rather accurate predictions were reported for various binary drug–target benchmark datasets. However, a notable drawback of a binary representation of interaction data is that missing endpoints for non-interacting drug–target pairs are not differentiated from inactive cases, and that predicted levels of activity depend on pre-defined binarization thresholds. In this paper, we present a method called SimBoost that predicts continuous (non-binary) values of binding affinities of compounds and proteins and thus incorporates the whole interaction spectrum from true negative to true positive interactions. Additionally, we propose a version of the method called SimBoostQuant which computes a prediction interval in order to assess the confidence of the predicted affinity, thus defining the Applicability Domain metrics explicitly. We evaluate SimBoost and SimBoostQuant on two established drug–target interaction benchmark datasets and one new dataset that we propose to use as a benchmark for read-across cheminformatics applications. We demonstrate that our methods outperform the previously reported models across the studied datasets.
At SIGMOD 2015, an article was presented with the title “DBSCAN Revisited: Mis-Claim, Un-Fixability, and Approximation” that won the conference’s best paper award. In this technical correspondence, we want to point out some inaccuracies in the way DBSCAN was represented, and why the criticism should have been directed at the assumption about the performance of spatial index structures such as R-trees and not at an algorithm that can use such indexes. We will also discuss the relationship of DBSCAN performance and the indexability of the dataset, and discuss some heuristics for choosing appropriate DBSCAN parameters. Some indicators of bad parameters will be proposed to help guide future users of this algorithm in choosing parameters such as to obtain both meaningful results and good performance. In new experiments, we show that the new SIGMOD 2015 methods do not appear to offer practical benefits if the DBSCAN parameters are well chosen and thus they are primarily of theoretical interest. In conclusion, the original DBSCAN algorithm with effective indexes and reasonably chosen parameter values performs competitively compared to the method proposed by Gan and Tao.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.