We report on the analysis of volatile compounds by SPME-GC-MS for individual roasted coffee beans. The aim was to understand the relative abundance and variability of volatile compounds between individual roasted coffee beans at constant roasting conditions. Twenty-five batches of Arabica and robusta species were sampled from 13 countries, and 10 single coffee beans randomly selected from each batch were individually roasted in a fluidised-bed roaster at 210 °C for 3 min. High variability (CV = 14.0–53.3%) of 50 volatile compounds in roasted coffee was obtained within batches (10 beans per batch). Phenols and heterocyclic nitrogen compounds generally had higher intra-batch variation, while ketones were the most uniform compounds (CV < 20%). The variation between batches was much higher, with the CV ranging from 15.6 to 179.3%. The highest variation was observed for 2,3-butanediol, 3-ethylpyridine and hexanal. It was also possible to build classification models based on geographical origin, obtaining 99.5% and 90.8% accuracy using LDA or MLR classifiers respectively, and classification between Arabica and robusta beans. These results give further insight into natural variation of coffee aroma and could be used to obtain higher quality and more consistent final products. Our results suggest that coffee volatile concentration is also influenced by other factors than simply the roasting degree, especially green coffee composition, which is in turn influenced by the coffee species, geographical origin, ripening stage and pre- and post-harvest processing.
HighlightsHSI was applied for non-destructive prediction of total protein content in wheat kernels.Above 2100 wheat kernels were taken from ~200 batches and individually analysed.PLS regression models had R2 = 0.82 and prediction error lower than 0.93%.Protein distribution had wide range (6–20%) and was visualised by applying the calibration.The performance of HgGcTe was superior to the one built by simulating InGaAs sensors.
Hyperspectral imaging (HSI) combines spectroscopy and imaging, providing information about the chemical properties of a material and their spatial distribution. It represents an advance of traditional Near-Infrared (NIR) spectroscopy. The present work reviews the most recent applications of NIR spectroscopy for cereal grain evaluation, then focuses on the use of HSI in this field. The progress of research from ground material to whole grains and single kernels is detailed. The potential of NIR-based methods to predict protein content, sprout damage and a-amylase activity in wheat and barley is shown, in addition to assessment of quality parameters in other cereals such as rice, maize and oats, and the estimation of fungal infection. This analytical technique also offers the possibility to rapidly classify grains based on properties such as variety, geographical origin, kernel hardness, etc. Further applications of HSI are expected in the near future, for its potential for rapid single-kernel analysis.
Hyperspectral imaging (HSI) is a novel technology for the food sector that enables rapid non-contact analysis of food materials. HSI was applied for the first time to whole green coffee beans, at a single seed level, for quantitative prediction of sucrose, caffeine and trigonelline content. In addition, the intra-bean distribution of coffee constituents was analysed in Arabica and Robusta coffees on a large sample set from 12 countries, using a total of 260 samples. Individual green coffee beans were scanned by reflectance HSI (980–2500 nm) and then the concentration of sucrose, caffeine and trigonelline analysed with a reference method (HPLC-MS). Quantitative prediction models were subsequently built using Partial Least Squares (PLS) regression. Large variations in sucrose, caffeine and trigonelline were found between different species and origin, but also within beans from the same batch. It was shown that estimation of sucrose content is possible for screening purposes (R2 = 0.65; prediction error of ~ 0.7% w/w coffee, with observed range of ~ 6.5%), while the performance of the PLS model was better for caffeine and trigonelline prediction (R2 = 0.85 and R2 = 0.82, respectively; prediction errors of 0.2 and 0.1%, on a range of 2.3 and 1.1% w/w coffee, respectively). The prediction error is acceptable mainly for laboratory applications, with the potential application to breeding programmes and for screening purposes for the food industry. The spatial distribution of coffee constituents was also successfully visualised for single beans and this enabled mapping of the analytes across the bean structure at single pixel level.
HighlightsMeasurements of single cocoa beans were made by NIR HSI.PLS regression models were built for several chemical properties.Fermentation (FI), total phenolics (TP) and antioxidant activity (AA) were predicted.Prediction performance was suitable for screening purposes.
The presence of a few kernels with sprouting problems in a batch of wheat can result in enzymatic activity
sufficient to compromise flour functionality and bread quality. This is commonly assessed using the Hagberg Falling
Number (HFN) method, which is a batch analysis. Hyperspectral imaging (HSI) can provide analysis at the single grain level
with potential for improved performance. The present paper deals with the development and application of calibrations
obtained using an HSI system working in the near infrared (NIR) region (~900–2500 nm) and reference measurements of
HFN. A partial least squares regression calibration has been built using 425 wheat samples with a HFN range of 62–318 s,
including field and laboratory pre-germinated samples placed under wet conditions. Two different approaches were
tested to apply calibrations: i) application of the calibration to each pixel, followed by calculation of the average of the
resulting values for each object (kernel); ii) calculation of the average spectrum for each object, followed by application of
the calibration to the mean spectrum. The calibration performance achieved for HFN (R2 = 0.6; RMSEC ~ 50 sRMSEP ~ 63 s)
compares favourably with other studies using NIR spectroscopy. Linear spectral pre-treatments lead to similar results
when applying the two methods, while non-linear treatments such as standard normal variate showed obvious
differences between these approaches. A classification model based on linear discriminant analysis (LDA) was also
applied to segregate wheat kernels into low (<250 s) and high (>250 s) HFN groups. LDA correctly classified 86.4% of
the samples, with a classification accuracy of 97.9% when using an HFN threshold of 150 s. These results are promising
in terms of wheat quality assessment using a rapid and non-destructive technique which is able to analyse wheat
properties on a single-kernel basis, and to classify samples as acceptable or unacceptable for flour production.
Hyperspectral imaging (1000–2500 nm) was used for rapid prediction of moisture and total lipid content in intact green coffee beans on a single bean basis. Arabica and Robusta samples from several growing locations were scanned using a “push-broom” system. Hypercubes were segmented to select single beans, and average spectra were measured for each bean. Partial Least Squares regression was used to build quantitative prediction models on single beans (n = 320–350). The models exhibited good performance and acceptable prediction errors of ∼0.28% for moisture and ∼0.89% for lipids.This study represents the first time that HSI-based quantitative prediction models have been developed for coffee, and specifically green coffee beans. In addition, this is the first attempt to build such models using single intact coffee beans. The composition variability between beans was studied, and fat and moisture distribution were visualized within individual coffee beans. This rapid, non-destructive approach could have important applications for research laboratories, breeding programmes, and for rapid screening for industry.
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