& the Expedition 302 Scientists †The Palaeocene/Eocene thermal maximum, ,55 million years ago, was a brief period of widespread, extreme climatic warming [1][2][3] , that was associated with massive atmospheric greenhouse gas input 4 . Although aspects of the resulting environmental changes are well documented at low latitudes, no data were available to quantify simultaneous changes in the Arctic region. Here we identify the Palaeocene/Eocene thermal maximum in a marine sedimentary sequence obtained during the Arctic Coring Expedition 5 . We show that sea surface temperatures near the North Pole increased from ,18 8C to over 23 8C during this event. Such warm values imply the absence of ice and thus exclude the influence of ice-albedo feedbacks on this Arctic warming. At the same time, sea level rose while anoxic and euxinic conditions developed in the ocean's bottom waters and photic zone, respectively. Increasing temperature and sea level match expectations based on palaeoclimate model simulations 6 , but the absolute polar temperatures that we derive before, during and after the event are more than 10 8C warmer than those model-predicted. This suggests that higher-than-modern greenhouse gas concentrations must have operated in conjunction with other feedback mechanisms-perhaps polar stratospheric clouds 7 or hurricane-induced ocean mixing 8 -to amplify early Palaeogene polar temperatures.

A study of upper Paleocene-lower Eocene (P-E) sediments deposited on the Lomonosov Ridge in the central Arctic Ocean reveals relatively high abundances of terrestrial biomarkers. These include dehydroabietane and simonellite derived from conifers (gymnosperms) and a tetra-aromatic triterpenoid derived from angiosperms. The relative percentage of the angiosperm biomarker of the summed angiosperm + conifer biomarkers was increased at the end of the Paleocene-Eocene thermal maximum (PETM), different when observed with pollen counts which showed a relative decrease in angiosperm pollen. Stable carbon isotopic analysis of these biomarkers shows that the negative carbon isotope excursion (CIE) during the PETM amounts to 3‰ for both conifer biomarkers, dehydroabietane and simonellite, comparable to the magnitude of the CIE inferred from marine carbonates, but significantly lower than the 4.5‰ of the terrestrial C 29 n-alkane [M.

We have applied a recently introduced proxy, the BIT (branched and isoprenoid tetraether) index, to determine terrestrial organic matter (TOM) transport from the rivers Rhine and Meuse and their tributaries to the southern North Sea. This index is based on crenarchaeol, an isoprenoidal glycerol dialkyl glycerol tetraether (GDGT) predominantly derived from aquatic Crenarchaeota and branched GDGTs produced by soil bacteria. Up to 1.6 ng L 21 of branched GDGTs were measured in seawater, demonstrating the presence of TOM in the southern North Sea. BIT indices were inversely correlated with salinity, indicating that this TOM was recently supplied by rivers. The substantial amount of branched GDGTs (35 ng L 21 ) detected in river water and the high branched GDGT concentration measured in the water of a Swiss peat bog (5,900 ng L 21 ) suggest fluvial transport of branched GDGTs from peats and soils to the oceans. The high crenarchaeol concentration measured in river water (4.4 ng L 21 ) was probably derived from crenarchaeota living in rivers and organic material from soil and peat. The BIT index, d 13 C value, and C : N ratio of surface sediments deposited in the southern North Sea were compared to determine TOM deposition. BIT index (0.07 to 0.26) and d 13 C (220.6 to 222.9%) both showed substantial small-scale differences in TOM deposition in the southern North Sea, but this pattern was not obvious from the C : N ratios. A good correlation was found between d 13 C and branched GDGT concentrations, indicating that the absolute GDGT concentrations give additional information to the BIT index.Rivers provide large amounts of organic carbon to the marine environment (,4 3 10 14 g C yr 21 ; Schlesinger and Melack 1981) and are thus a key component of the global carbon cycle. Analysis of the terrestrial organic matter (TOM) buried in marine sediments is widely carried out to estimate the importance and fate of TOM in carbon cycling. In paleoenvironmental studies, determination of TOM transport can also provide useful information on, for example, the proximity of the continent, river fluxes, and wind strength. The distinction between organic carbon of marine and terrestrial origins is commonly made by analyzing the C : N ratio and the 13 C content of bulk organic matter (Meyers et al. 1994). Numerous biomarkers derived from higher plants are also used as proxies for tracing TOM input in marine sediments. However, several problems are inherent to both approaches (Hedges et al. 1997;Pancost and Boot 2004). For instance, the inconstancy of the d 13 C values of phytoplanktonic organic matter and TOM (e.g., C3 vs. C4 vegetation) renders the estimation of the proportion of terrestrial to marine organic matter in sediments difficult. Similarly, the difference in degradation rates of terrestrial-derived and marine-derived compounds may lead to erroneous estimation of terrestrial input in marine settings. These shortcomings make accurate quantification of TOM input in marine environments challenging (Hopmans et al. 2004, an...

Branched glycerol dialkyl glycerol tetraether (GDGT) distributions observed in a sediment core from Lake McKenzie were utilized to quantitatively reconstruct the pattern of mean annual air temperature (MAAT) from coastal subtropical eastern Australia between 37 and 18.3 cal ka BP and 14.0 cal ka BP to present. Both the reconstructed trend and amplitude of MAAT changes from the top of the sediment core were nearly identical to a local instrumental MAAT record from Fraser Island, providing confidence that in this sediment core branched GDGTs could be used to produce a quantitative record of past MAAT. The reconstructed trend of MAAT during 37 to 18.3 cal ka BP and timing of the Last Glacial Maximum (LGM) in the Lake McKenzie record were in agreement with previously published nearby marine climate records. The amplitude of lower-than-present MAAT during the LGM potentially provides information on the latitude of separation of the Tasman Front from the East Australian current in the subtropical western Pacific. The Lake McKenzie record shows an earlier onset of near modern day warm temperatures in the early Holocene compared to marine records and the presence of a warmer than present day period during the mid-Holocene.

[2] Two commonly used proxies based on the distribution of glycerol dialkyl glycerol tetraethers (GDGTs) are the TEX 86 (TetraEther indeX of 86 carbon atoms) paleothermometer for sea surface temperature reconstructions and the BIT (Branched Isoprenoid Tetraether) index for reconstructing soil organic matter input to the ocean. An initial round-robin study of two sediment extracts, in which 15 laboratories participated, showed relatively consistent TEX 86 values (reproducibility 63-4 C when translated to temperature) but a large spread in BIT measurements (reproducibility 60.41 on a scale of 0-1). Here we report results of a second round-robin study with 35 laboratories in which three sediments, one sediment extract, and two mixtures of pure, isolated GDGTs were analyzed. The results for TEX 86 and BIT index showed improvement compared to the previous round-robin study. The reproducibility, indicating interlaboratory variation, of TEX 86 values ranged from 1.3 to 3.0 C when translated to temperature. These results are similar to those of other temperature proxies used in paleoceanography. Comparison of the results obtained from one of the three sediments showed that TEX 86 and BIT indices are not significantly affected by interlaboratory differences in sediment extraction techniques. BIT values of the sediments and extracts were at the extremes of the index with values close to 0 or 1, and showed good reproducibility (ranging from 0.013 to 0.042). However, the measured BIT values for the two GDGT mixtures, with known molar ratios of crenarchaeol and branched GDGTs, had intermediate BIT values and showed poor reproducibility and a large overestimation of the ''true'' (i.e., molarbased) BIT index. The latter is likely due to, among other factors, the higher mass spectrometric response of branched GDGTs compared to crenarchaeol, which also varies among mass spectrometers. Correction for this different mass spectrometric response showed a considerable improvement in the reproducibility of BIT index measurements among laboratories, as well as a substantially improved estimation of molar-based BIT values. This suggests that standard mixtures should be used in order to obtain consistent, and molar-based, BIT values.