Starch synthesis in leaves was increased by phosphate starvation or by treatments which decreased cytoplasmic orthophosphate levels (such as mannose feeding). Usally less than 30% of the total carbon fixed during CO2 assimlation was incorporated into starch in spinach (Spinacia oleracca L.), spinach beet (Beta vulgaris), and tobacco (Nicotiana tabacum) leaves.In isolated spinach chloroplasts, formation of starch from CO2 was usualy less than in leaves.

The labeling of intermediate compounds and photosynthetic cofactors during photosynthesis and periods of darkness by Chlorella pyrenoidosa in the presence of 32P‐labeled phosphate and 14CO2 have heen investigated. Algae adapted to photosynthesis in air were used, and the level of carbon dioxide was maintained at approximately 0.04 % and at constant specific radioactivity during the course of the experiments. The transient changes which occur in the levels of labeled fructose‐1,6‐diphosphate and in sedoheptulose‐1,7‐diphosphate, and in the corresponding monophosphates when the light is turned off suggest a light activation of the diphosphatase enzymes which decays after about 2 minutes of darkness. It is suggested that a light‐dark switch in enzymic activities permits photosynthesis and glycolysis to occur in light and dark respectively with the same enzymic apparatus. The greatly diminished rate of disappearanec of the carboxylation substrate, ribulose‐1,5‐diphosphate, after about 2 minutes suggests that there is also a light activation of the carboxylation reaction in vivo. Large transient changes in the level of pyrophosphate between light and dark indicate that there may be an unstable cofactor which decomposes to give pyrophosphate during or alter killing of the algal cells. The possibility that this cofactor is involved in an activation of Carbon dioxide for the carboxylation reaction in vivo is suggested. Light‐dark transient changes in labeling of other compounds of the photosynthetic carbon reduction cycle and related compounds were determined, and possible significance of these changes is discussed.(PDF DAMAGED)

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