The behavior of oligonucleotides containing 5-iodouracil, 5-bromocytidine, and 5-iodocytidine in concentrated ammonia is described. 5-Aminouracil and 5-aminocytidine are obtained as side products when deprotection is performed at 60 degrees C. Small amounts, if any, of side products are obtained when ammonia deprotection is performed at room temperature. The base-pairing properties of these 5-halopyrimidines including triple helix are described.
Coll(salen)t derivatives whose ethanediyl moiety carries an o-alkenyl side chain react with oxygen and methanol or ethanol t o give, via interaction between intermediate Colll species and the pendant olefin, alkylated products with a P-alkoxy substituted carbon bridge between cobalt and the equatorial ligand; the corresponding intermolecular reaction between Coll(salen) and hex-I-ene does not take place.
SummaryThe preparation of peptide nucleic acids (PNA) carrying a c-myc tag-peptide sequence is described. These PNApeptide chimeras have higher affinity to complementary DNA than unmodified oligonucleotides. Moreover, they can be used as nonradioactive probes with sensitivity similar to other nonradioactive methods.
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