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Abstract— A highly active in vitro protein synthesizing system (S‐28) has been prepared from rat brain. Poly (U)‐dependent [3H] phenylalanine incorporation by brain S‐28 system is significantly inhibited by D‐amphetamine. The extent of inhibition by amphetamine is significantly higher than by other biogenic amines such as dopamine and serotonin. At the 100°g level of amphetamine, the inhibition is about 70°. Experiments with ribosomes and soluble enzymes from control and amphetamine‐treated systems indicate that the observed inhibition may be due to the effect of the drug on the ribosomes. Kinetic analysis of the reaction mixture in the presence as well as absence of D‐amphetamine indicate that this sympathomimetic drug inhibits polysome formation in vitro.

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