As a highly sensitive food, the safety of meat is an important issue in the context of food safety. The current analytical methods for detection are based on different, invasive proce-dures. The analysis of these foods therefore causes avoidable food losses in the case of foods that are still suitable for consumption. Spatially offset Raman spectroscopy (SORS) is a po-tential alternative analytical method, as it offers the possibility to measure through packag-ing. Using packaged chicken breast as an example, we simulated two different storage condi-tions and compared these samples to control samples using SORS. To validate the group as-signment and to monitor the resulting changes, total bacterial count and NMR spectra of the meat extracts were measured and evaluated. We overcame the various difficulties in the multivariate data evaluation of the through package measured Raman spectra and were able to classify samples deviating from the control group with very high accuracies and simulta-neous absence of false positive results.
The determination of fish species is an important task for food control, especially in the case of very similar and processed fish or fish products. Typically, this analysis is carried out by DNA-based methods. However, especially for small sample series the effort for sample preparation and the required analysis time are not satisfactory. We show that by means of a simple extraction and analysis of the 1H NMR spectra of these extracts a fast discrimination of fish species is possible. For this purpose, 69 samples consisting of five sample groups iridescent shark, European plaice, rock sole, witch flounder and common sole were analysed. The large differences in the 1H spectra could be further highlighted with a principal component and led already to a graphical differentiation of most sample groups. The performed classification of the samples using a subspace discriminant algorithm showed a recognition rate of 100% for all samples, regardless of additives like citric acid or lysine. These results show that 1H NMR spectroscopy is a robust, simple and rapid method for the recognition of the fish species studied.
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