No abstract

No abstract

Abstract. A method for isolating protoplasts from leaves of the submerged aquatic angiosperm Potamogeton lucens L. is described. The protoplasts are produced enzymatically from leaf strips using 1.5% (w/v) Cellulysin, 0.3% (w/v) Macerozyme R10, and 2.5% (v/v) β‐glucuronidase at 27°C in the dark. Subsequently the protoplasts are purified on a discontinuous Ficoll gradient. The yield obtained is approximately 20% of the starting material on a chlorophyll basis. The viability is high, namely more than 90% as estimated with Evans Blue. Cells of the intact leaves of P. lucens can use HCO3− for photosynthesis. 14CO2 fixation experiments at pH 6.0 and 8.5 suggest that these isolated protoplasts can also use bicarbonate as a carbon source.

In this paper, we report on the results of the CHI98 workshop on task, process and work analysis coupled with object modeling. This workshop was a follow-up to a CHI97 workshop of the same topic. This year's workshop took as its starting point the summary paper and framework created in last year's workshop. The goal of this year's workshop was to bridge the conceptual gulf between current HCI practice and current development practice. The result of this workshop is a proposed set of extensions to UML, a key standard in the objectoriented development community.