The direct involvement of the human leukocyte antigen class II DR-DQ genes in type 1 diabetes (T1D) is well established, and these genes display a complex hierarchy of risk effects at the genotype and haplotype levels. We investigated, using data from 38 studies, whether the DR-DQ haplotypes and genotypes show the same relative predispositional effects across populations and ethnic groups. Significant differences in risk within a population were considered, as well as comparisons across populations using the patient/control (P/C) ratio. Within a population, the ratio of the P/C ratios for two different genotypes or haplotypes is a function only of the absolute penetrance values, allowing ranking of risk effects. Categories of consistent predisposing, intermediate ('neutral'), and protective haplotypes were identified and found to correlate with disease prevalence and the marked ethnic differences in DRB1-DQB1 frequencies. Specific effects were identified, for example for predisposing haplotypes, there was a statistically significant and consistent hierarchy for DR4 DQB1*0302s: DRB1*0405 ¼ *0401 ¼ *0402 > *0404 > *0403, with DRB1*0301 DQB1*0200 (DR3) being significantly less predisposing than DRB1*0402 and more than DRB1*0404. The predisposing DRB1*0401 DQB1*0302 haplotype was relatively increased compared with the protective haplotype DRB1*0401 DQB1*0301 in heterozygotes with DR3 compared with heterozygotes with DRB1*0101 DQB1*0501 (DR1). Our results show that metaanalyses and use of the P/C ratio and rankings thereof can be valuable in determining T1D risk factors at the haplotype and amino acid residue levels.

a b s t r a c tThe prehistoric establishment and expansion of permanent settlements on the Northern Channel Islands of southern California generally follows a pattern predicted by the population ecology model, the ideal free distribution (IFD). We determine this by comparing the abundant archaeological record of these Islands against a careful quantification of habitat suitability using areal photography, satellite imagery, and field studies. We assess watershed area, length of rocky intertidal zone, length of sandy beach for plank canoe pull-outs and area of off-shore kelp beds, for 46 coastal locations. A simple descriptive analysis supports key IFD predictions. A Bayesian model fitted with the Gibbs sampler allows us to reconstruct the Native assessment of habitat that appears to underlie this process. Use of the Gibbs sampler mitigates the impact of missing data, censored variables, and uncertainty in radiocarbon dates; it allows us to predict where new settlements may yet be discovered. Theoretically, our results support a behavioral ecology interpretation of settlement history, human population expansion, and economic intensification in this region. They also demonstrate Bayesian analytical methods capable of making full use of the information available in archaeological datasets.Ó 2010 Elsevier Inc. All rights reserved. Introduction and problemThe Native Americans who moved from the mainland to settle the Northern Channel Islands of southern California knew well the country they were settling. By first recorded permanent settlement ($8000-7000 cal. years BP) they had been exploring and seasonally exploiting the resources of the islands for as much as 5000 years Kennett, 2005;. Subsequent to that first settlement further Island colonization of coastal sites at the mouths of major drainages drew on local knowledge gained through even more lengthy experience. Settlers were familiar with the suitability of the habitats they were about to occupy and, we might predict, they established residential sites in an orderly process of adaptive decision-making: Settle first in the most salubrious location. When, with growing exploitation or crowding, its resources were depressed and its value declined to match the next-ranked locale, establish a new settlement there. As population grew this process repeated, adding further new settlements in locations ordered by habitat suitability. In parallel, we expect overall quality of life in all occupied locales declined due to reductions in the availability of or access to critical resources. This process is neatly captured in a behavioral ecology model: the ideal free distribution (IFD, Fretwell and Lucas, 1969). Analysis of environmental and archaeological data from the Northern Channel Islands indicates that colonization there follows IFD predictions. To demonstrate this, we first describe the prehistory of the Northern Channel Islands. We then introduce the IFD, with the goal of predicting how population growth, intensified use and declining suitability generate a predictab...

While the vast majority of genome size variation in plants is due to differences in repetitive sequence, we know little about how selection acts on repeat content in natural populations. Here we investigate parallel changes in intraspecific genome size and repeat content of domesticated maize (Zea mays) landraces and their wild relative teosinte across altitudinal gradients in Mesoamerica and South America. We combine genotyping, low coverage whole-genome sequence data, and flow cytometry to test for evidence of selection on genome size and individual repeat abundance. We find that population structure alone cannot explain the observed variation, implying that clinal patterns of genome size are maintained by natural selection. Our modeling additionally provides evidence of selection on individual heterochromatic knob repeats, likely due to their large individual contribution to genome size. To better understand the phenotypes driving selection on genome size, we conducted a growth chamber experiment using a population of highland teosinte exhibiting extensive variation in genome size. We find weak support for a positive correlation between genome size and cell size, but stronger support for a negative correlation between genome size and the rate of cell production. Reanalyzing published data of cell counts in maize shoot apical meristems, we then identify a negative correlation between cell production rate and flowering time. Together, our data suggest a model in which variation in genome size is driven by natural selection on flowering time across altitudinal clines, connecting intraspecific variation in repetitive sequence to important differences in adaptive phenotypes.

Evaluations of the success of different conservation strategies are still in their infancy. We used four different measures of project outcomes-ecological, economic, attitudinal, and behavioral-to Palabras Clave: acceso al mercado, conservación y desarrollo integrados, descentralización, monitoreo de la conservación, proteccionismo

Agricultural foods and technologies are thought to have eased the mechanical demands of diet-how often or how hard one had to chew-in human populations worldwide. Some evidence suggests correspondingly worldwide changes in skull shape and form across the agricultural transition, although these changes have proved difficult to characterize at a global scale. Here, adapting a quantitative genetics mixed model for complex phenotypes, we quantify the influence of diet on global human skull shape and form. We detect modest directional differences between foragers and farmers. The effects are consistent with softer diets in preindustrial farming groups and are most pronounced and reliably directional when the farming class is limited to dairying populations. Diet effect magnitudes are relatively small, affirming the primary role of neutral evolutionary processes-genetic drift, mutation, and gene flow structured by population history and migrations-in shaping diversity in the human skull. The results also bring an additional perspective to the paradox of why Homo sapiens, particularly agriculturalists, appear to be relatively well suited to efficient (high-leverage) chewing.foragers | farmers | subsistence effects | human skull form | mastication T he emergence and spread of agriculture are among the more remarkable developments in the evolutionary history of Homo sapiens. This change in lifeway appears to be associated with changes in human skull shape and form. Although global cranial diversity is generally well explained by neutral evolutionary processes (1-4), early farmers tend to have a chewing architecture that is, at least in some dimensions, less massive than that of their hunter-gatherer counterparts (refs. 5-20 and 21, chap. 7). Explanatory scenarios cohere around the idea that softer agricultural foods reduce masticatory demands, resulting in less robust craniofacial skeletons and reduced and repositioned chewing muscles. This is the essence of the "masticatory-functional hypothesis" Carlson and Van Gerven (5) posited four decades ago to explain morphological differences among a chronological series of ancient Nubian populations-from Mesolithic hunter-gatherers to Christian agriculturalists. Subsequent forager-farmer comparisons for European, Asian, and American samples support a trend of craniofacial reduction with agriculture (7-19). Most of these studies sample a small number of geographically local populations (but see refs. 22 and 23). Local comparisons are valuable because they often provide a detailed picture of the cultural, dietary, and chronological context for the morphological differences between closely related groups. In some cases, cultural and other evidence supports a hypothesis of biological continuity between the foragers and descendant farmers (5-7, 10, 12, 14, 16-19). However, with few sampled groups, it can be difficult to separate diet effects from other factors differentiating the populations. Moreover, the major dimensions of reduction can vary from study to study, and some farmer ...

Use-wear analysis provides a means of studying traces produced on animal bone during manufacture and use in an effort to reconstruct these processes. Often, these analyses are qualitative and based on experience and expertise. Previous studies have focused on interpreting final traces, but little is known about how these traces develop and change over time. We propose the use of an innovative quantitative method for studying bone surface traces that aims to reduce any unreliable or non-replicable results that can confound more traditional qualitative analyses. We seek to understand the basics of use-wear formation over Time by taking incremental molds of bone specimens subjected to a controlled, mechanical experiment. This study assesses how bone wears during extended use on three Material types (fresh skin, processed leather, or dry bark), from three initial Manufacturing states (unworked, ground with sandstone, or scraped with flint). With data obtained from a confocal disc-scanning microscope, we then apply 3D surface texture analysis using ISO 25178 parameters: surface roughness [Sa], autocorrelation length [Sal], peak curvature [Spc], and upper material ratio [Smr1]. We employ a multilevel multivariate Bayesian model to explain parameter variation under experimental conditions. Our findings show how duration of use strongly affects the transformation of the bone’s surface. Unworked bone is completely distinguishable from bone used for long time intervals and those modified by scraping. Interestingly, material wear does not often produce type-specific traces, but does affect the rate of bone alteration and how it is transformed. Specifically, fresh skin transforms bone at a faster rate than other materials. This novel quantitative and experimental approach enhances our understanding of the use of bone as a raw material for making and using tools and provides a foundation for future exploration of archaeological materials and questions.

Conservation strategies to protect biodiversity and support household livelihoods face numerous challenges. Across the tropics, efforts focus on balancing trade-offs in local communities near the borders of protected areas. Devolving rights and control over certain resources to communities is increasingly considered necessary, but decades of attempts have yielded limited success and few lessons on how such interventions could be successful in improving livelihoods. We investigated a key feature of household well-being, the experience of food insecurity, in villages across Tanzania's northern wildlife tourist circuit. Using a sample of 2,499 primarily livestock-keeping households we compared food insecurity in villages participating in the country's principal community-based conservation strategy with nearby control areas. We tested whether community-based projects could offset the central costs experienced by households near strictly protected areas (i.e. frequent human–wildlife conflict and restricted access to resources). We found substantial heterogeneity in outcomes associated with the presence of community-based conservation projects across multiple project sites. Although households in project villages experienced more frequent conflict with wildlife and received few provisioned benefits, there is evidence that these households may have been buffered to some degree against negative effects of wildlife conflict. We interpret our results in light of qualitative institutional factors that may explain various project outcomes. Tanzania, like many areas of conservation importance, contains threatened biodiversity alongside areas of extreme poverty. Our analyses highlight the need to examine more precisely the complex and locally specific mechanisms by which interventions do or do not benefit wildlife and local communities.

Previous studies demonstrated the existence of a relationship between climate and cranial form. The mixed model quantifies this relationship concretely. Evolutionary questions that require population structure and phylogeny to be disentangled from potential drivers of selection may be particularly well addressed by mixed models. Am J Phys Anthropol 160:593-603, 2016. © 2015 Wiley Periodicals, Inc.