Advances in the field of quantum sensing mean that magnetic field sensors, operating at room temperature, are now able to achieve sensitivity similar to that of cryogenically cooled devices (SQUIDs). This means that room temperature magnetoencephalography (MEG), with a greatly increased flexibility of sensor placement can now be considered. Further, these new sensors can be placed directly on the scalp surface giving, theoretically, a large increase in the magnitude of the measured signal. Here, we present recordings made using a single optically-pumped magnetometer (OPM) in combination with a 3D-printed head-cast designed to accurately locate and orient the sensor relative to brain anatomy. Since our OPM is configured as a magnetometer it is highly sensitive to environmental interference. However, we show that this problem can be ameliorated via the use of simultaneous reference sensor recordings. Using median nerve stimulation, we show that the OPM can detect both evoked (phase-locked) and induced (non-phase-locked oscillatory) changes when placed over sensory cortex, with signals ~4 times larger than equivalent SQUID measurements. Using source modelling, we show that our system allows localisation of the evoked response to somatosensory cortex. Further, source-space modelling shows that, with 13 sequential OPM measurements, source-space signal-to-noise ratio (SNR) is comparable to that from a 271-channel SQUID system. Our results highlight the opportunity presented by OPMs to generate uncooled, potentially low-cost, high SNR MEG systems.
HighlightsWe propose a method for constructing flexible head-casts to stabilize the head during MEG scanning.Co-registration error is minimized by using MRI images to pre-define fiducial coil locations.Within- and between-session movement is <0.25 and <1 mm respectively.This enables high reproducibility of source level results.
Immunohistochemistry (IHC) combined with fluorescence microscopy provides an important and widely used tool for researchers and pathologists to image multiple biomarkers in tissue specimens. However, multiplex IHC using standard fluorescence microscopy is generally limited to 3–5 different biomarkers, with hyperspectral or multispectral methods limited to 8. We report the development of a new technology based on novel photocleavable mass-tags (PC-MTs) for facile antibody labeling, which enables highly multiplexed IHC based on MALDI mass spectrometric imaging (MALDI-IHC). This approach significantly exceeds the multiplexity of both fluorescence- and previous cleavable mass-tag-based methods. Up to 12-plex MALDI-IHC was demonstrated on mouse brain, human tonsil, and breast cancer tissues specimens, reflecting the known molecular composition, anatomy, and pathology of the targeted biomarkers. Novel dual-labeled fluorescent PC-MT antibodies and label-free small-molecule mass spectrometric imaging greatly extend the capability of this new approach. MALDI-IHC shows promise for use in the fields of tissue pathology, tissue diagnostics, therapeutics, and precision medicine.
This study examined changes in the biomechanical properties of cultured pulmonary microvascular endothelial cells (ECs) and neutrophils induced by adhesion of neutrophils to these ECs. The biomechanical properties of cells were evaluated using magnetic twisting cytometry, which measures the angular rotation of ferromagnetic beads bound to cells through antibody ligation on application of a specified magnetic torque. Adhesion of neutrophils to 24-hour tumor necrosis factor-␣ (TNF-␣)-treated ECs, but not to untreated ECs, induced an increase in EC stiffness within 2 minutes, which was accompanied by an increase and a reorganization of F-actin in ECs. A cell-permeant, phosphoinositide-binding peptide attenuated the EC stiffening response, suggesting that intracellular phosphoinositides are required. The stiffening response was not inhibited by ML-7, a myosin light-chain kinase inhibitor, or BAPTA, an intracellular Ca 2؉ chelator. Moreover, the phosphorylation pattern of the regulatory myosin light chains was unaltered within 15 minutes of neutrophil adherence. These data suggested that the EC stiffening response appeared not to be mediated by myosin light-chain-dependent mechanisms. Concomitantly, neutrophil adhesion to 24-hour TNF-␣-treated ECs also induced changes in the biomechanical properties of neutrophils compared to neutrophils bound to untreated ECs. Taken together, these results demonstrated that neutrophil adhesion to TNF-␣-treated ECs induces changes in the biomechanical properties of both cell types through actin cytoskeletal remodeling. These changes may modulate neutrophil transmigration across the endothelium during inflammation. IntroductionNeutrophil transmigration across the vascular endothelium is a highly regulated process that requires the up-regulation of neutrophil and endothelial cell (EC) adhesion molecules. 1 During neutrophil adhesion to ECs, binding of the neutrophil or EC adhesion molecules to their ligands may induce intracellular signaling pathways and downstream events, which may in turn modulate neutrophil transmigration. Indeed, the ligation of various adhesion molecules can initiate signal transduction pathways and induce subsequent cellular changes. Cross-linking of neutrophil CD11/CD18 induces intracellular Ca 2ϩ increases, CD11/ CD18 up-regulation, and F-actin polymerization. 2 Ligation of EC E-selectin, P-selectin, ICAM-1, and VCAM-1 by antibodies induces transient increases in cytosolic Ca 2ϩ . [3][4][5] Similarly, neutrophil adherence and emigration also induce increases in intracellular Ca 2ϩ levels in ECs. 3,6 One of the downstream targets of these signaling events in ECs after neutrophil adherence is the actin cytoskeleton. Neutrophil adherence induces stress fiber formation in ECs, 3,7 and inhibition of the F-actin changes and actin polymerization in ECs reduces neutrophil transmigration as observed both in vivo and in vitro. [7][8][9] However, the mechanisms involved in mediating the EC actin cytoskeletal changes on neutrophil adhesion are not understood.In the system...
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