Washed cell preparations of Pseudomonas aureofaciens synthesize phenazine-lcarboxylic acid in a medium consisting of 0.2 M glycerol and 0.02 M D,L-lysine. Experiments with Ci4-labeled substrates indicate that all of the carbon atoms in the phenazine-1-carboxylic acid are derived from glycerol. Anthranilic acid inhibits synthesis of this phenazine compound. Incorporation of label from H3-shikimic acid has been demonstrated. In addition, a decrease in the incorporation of label from glycerol-l ,3-Cl4 into phenazine-1-carboxylic acid has been demonstrated in the presence of unlabeled shikimic acid.
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