These results indicate that CsA is directly toxic to LLC-PK1 cells with reduced DNA synthesis and cell cycle blockade. The mode of cell death, namely apoptosis or necrosis, is dose dependent. Fas may be an important mediator of CsA induced apoptosis in renal proximal tubular cells.

Core Ideas Cover crops were successfully established in corn with a drill interseeder.Cover crop biomass production varied notably across the mid‐Atlantic region.Spring cover crop biomass was often proportional to fall cover crop performance.Interseeding cover crops at corn growth stages V2–V3 decreased corn grain yields.Interseeding cover crops at or after corn V4 did not affect corn grain yield. Cover crop adoption remains low in the mid‐Atlantic United States despite potential conservation and production benefits. The short growing season window after corn (Zea mays L.) is a primary limiting factor. A high‐clearance grain drill was recently developed to allow for cover crop interseeding into standing cash crops. Experiment 1 tested the viability of drill interseeding cover crops into corn at the V5 growth stage across multiple locations. Experiment 2 tested interseeding timing (V2–V6 corn growth stage) on corn yield in Pennsylvania. At 16 locations throughout Maryland, Pennsylvania, and New York, we evaluated cover crop fall and spring biomass and the effect on corn yield. Cover crop treatments included annual ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot]), a mixture of legume species, and an annual ryegrass–legume mixture. Each cover crop treatment successfully established across locations yet was highly variable. Across locations, annual ryegrass–legume mixture produced the highest mean aboveground biomass in fall and spring. Spring biomass of interseeded cover crops generally increased compared with fall biomass. Interseeded cover crops did not affect grain yields of the host corn crop during the year of establishment across locations. Experiment 2 indicated that cover crops interseeded before the V3 growth stage reduced corn grain yields. We recommend interseeding at or after V4 to prevent competition with corn. Our results highlight the viability of drill‐interseeding as a strategy for increasing cover crop adoption.

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Objective Platelets play an important role in the pathophysiology of uteroplacental disease and platelet reactivity may be an important marker of uteroplacental disease activity. However, platelet reactivity has not been evaluated comprehensively in normal pregnancy. We sought to evaluate platelet reactivity using a number of agonists at defined time points in pregnancy using a novel platelet assay and compare these with a nonpregnant cohort.Design Prospective longitudinal study.Setting Outpatient department of a large tertiary referral centre.Sample Eighty participants with 30 nonpregnant women and 50 pregnant women assessed longitudinally.Methods This was a prospective cohort study performed longitudinally throughout uncomplicated singleton pregnancies with participants recruited before 15 weeks of gestation. They were controlled for a number of factors known to affect platelet reactivity. Blood samples were obtained in each trimester. Thirty nonpregnant healthy female volunteers also had a platelet assay performed. A modification of standard light transmission aggregometry was used to assess platelet function, with light absorbance measured following the addition of five different agonists at submaximal concentrations. Dose-response curves were plotted for each agonist for the nonpregnant cohort and in each trimester for the pregnant cohort.Main outcome measures Dose-response curves and median effective concentration.Results When compared with the nonpregnant controls a significant reduction was demonstrated in platelet reactivity to collagen during the first trimester of pregnancy (P < 0.0001). Platelet aggregation increased significantly from the first to third trimesters in response to collagen and arachidonic acid.Conclusion Platelet reactivity varies according to pregnancy state, gestational age and agonist. The finding that platelet reactivity is reduced in the first trimester of pregnancy may be useful for the interpretation of further studies examining the role of platelet reactivity in the first trimester of pregnancies that develop uteroplacental disease.

Our study confirms the reassuring prognosis for achieving a live birth in the unexplained RM population with a very low incidence of adverse events with the majority delivering appropriately grown fetuses at term.

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Red cell transfusion remains lower than international figures. This may form part of patient counselling when discussing the route of hysterectomy in the future.