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A novel system for on-line measurement of fat content in inhomogeneous pork trimmings is presented. The system allows near infrared (NIR) energy to interact with the meat using non-contact optics while it is travelling in large plastic boxes on a conveyor belt. A comparison was made between the log of the inverse of the interactance NIR spectra [log(1/T)], standard normal variate (SNV) and extended multiplicative signal correction (EMSC) as techniques for the correction of physical light scattering due to colour and textural differences, height variation and temperature fluctuations, depending on whether the meat was warm-cut or cold-cut. EMSC gave the best prediction results; a robust partial least squares regression using two factors resulted in a root mean square error (RMSEP) of 1.9% on 20 kg batches of inhomogeneous meat trimmings. The model was fully tested twice in an on-line environment at a slaughter house and performed with a RMSEP of 3.4% for a fat range of 8-55% in the first industrial trial and 2.82% in the second industrial trial.