Polyglycerol esters (PGEs), produced by esterification of fatty acids on polyglycerols, were analysed by High Resolution Mass Spectrometry (HRMS), HPLC-MS and U-HPLC-MS. A structural study of PGEs in 4 samples synthesised by the Gattefossé company was carried out using an elemental analysis of HRMS spectra and modelling of all probable isomers and cyclic structures. The results were used to construct a structural database of all species present in the 4 samples. After an assessment of the selectivity of 5 reversed phase columns: Aeris Widepore XB-C8, 3.6µm, 2.1x150 mm (Phenomenex), Acquity CSH C18 1.7µm 2.1x50mm, Acquity CSH Phenyl-Hexyl 1.7µm 2.1x50 mm, Acquity CSH Fluoro-Phenyl 1.7µm 2.1x50mm (Waters Co.) and Kinetex F5 1.7µm 2.1x100mm (Phenomenex), HPLC-MS and U-HPLC-MS analyses were performed on an Aeris Widepore XB-C8 (Phenomenex) column (HPLC) and Acquity CSH Fluoro-Phenyl (Waters) column (U-HPLC) with aqueous formic acid /acetonitrile in Highlights Batch comparisons and quantification of constituents of complex formulations Structural database construction with HRMS spectra and modelling of all probable PGEs
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