Estrogen receptors (ERs)are nuclear transcription factor receptorsthat play important roles in gene expression and cell cycle regulation. Because of their ligand-activated signaling implication in carcinogenesis,ERs are extensively researched as protein targets for anti-cancer drug discovery active in certain types of tumors. However, a major drawback is the emergence of wild type tumors withoverexpressed mutant variants of ER, which become resistant to estrogen inhibitor drugs. Herein we studied the binding mode and affinity of the semisynthetic estrogen agonist, 17-b-estradiol (E2) in normal and mutant variants of ERs, by means of molecular docking. Our results showed a small decrease in binding affinity, recorded in mutant variants of ERa and a change in the binding interactions formed when the compound wasdocked in an agonist-bound conformation of the ERa . Nevertheless we concluded that even if the binding affinity showed a small decrease in the case of mutant type receptors,E2 potency towards ER�won�t register a downward trend.
Numerous plants are currently used for both their curative and preventive effects in a wide range of pathologies. They possess multiple beneficial effects, including the well-known antioxidant capacity. Agrimonia eupatoria L. and Filipendula spp., from the Rosaceae family have emerged as a potential source of phenolic constituents, showing antioxidant activities. The aim of this study was to obtain ethanolic extracts from Agrimonia eupatoria L., Filipendula ulmaria (L.) Maxim. and Filipendula vulgaris Moench. collected from the West part of Romania and to determine their antioxidant capacity. Two DPPH assays were used to determine the antioxidant activity of the extracts, ethanolic solution of 1 and 0.1 mM DPPH. Our data indicated that all the extracts, regardless of the place of harvest possess significant antioxidant activity. The most potent activity was recorded for the extracts prepared from Agrimonia eupatoria L. collected from Bazia� � Locva Mountains in mixture with ethanolic solution of 0.1 mM DPPH, followed by Filipendula ulmaria (L.) Maxim, using the same concentration of ethanolic solution of standard antioxidant.
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