The use of arbuscular mycorrhizal fungi (AMF) in the production of rootstocks is an alternative to accelerate plant growth. However, their response depends on the symbionts species and environment. This study aimed at evaluating the influence of AMF species [Scutelospora heterogama, Gigaspora margarita, Glomus etunicatum, Acaulospora sp. and a control (non-inoculated)] on the vegetative development of citrus rootstocks {citrange 'Fepagro C37 Reck' [P. trifoliata (L.) Raf. x C. sinensis (L.) Osbeck.] and 'Kumquat' [Fortunella hindsii (L.) Swingle]}. The experimental design consisted of split-plot randomized blocks, with 10 plants per plot and 3 replications. Height, stem diameter, number of leaves, leaf area and fresh and dry root and shoot mass were evaluated. The colonization of AMF in the roots was also assessed, determining the percentage of colonization and density of hyphae, arbuscules and vesicles. The rootstocks showed no difference for the plant growth parameters, in the absence of AMF. The AMF species colonized the rootstocks roots, but were only effective in accelerating the citrange 'Fepagro C37 Reck' growth, especially when inoculated with Scutelospora heterogama, Gigaspora margarita and Glomus etunicatum. The influence of AMF on vegetative development depends on the citrus rootstock species.

RESúMEN -El miniestaquillado es un método eficaz y rápido de propagación vegetativa, empleado en plantas forestales y frutíferas, generando la formaçión de huertos homogéneos. Con el objetivo de determinar el manejo del abonado en la multiplicación de pitanguera bajo miniestaquillado en jardines clonales se próbaron dosis crecientes de Nitrógeno (N), trás dos etapas, en período de 260 días. Em la primera etapa se testaron las siguientes soluções: solución A: 10 g L -1 kristalon ® (NPk 4,(5)(6)(7)(8)(9)8), B: 10 g L -1 kristalon ® + 3,5g de urea (NPk 18-9,6-28,8) y c: 10 g L -1 kristalon ® + 7,0g de urea 8). En la segunda etapa se utilizó la mitad de las dosis del Kristalon ® (5g L -1 ) y las mismas de urea. Se evaluaron la producción y enraizamiento de las miniestaquillas y se monitoró los valores del pH y de la conductividad eléctrica (CE) de cada solución, además del sustrato. El diseño experimental fue completamente al azar con siete macetas teniendo cinco plantas madre cada una, por tratamiento. La primera recolección de miniestaquillas ocorrió a los siete dias traz empezar los tratamientos. Las soluciones A y B lograron mayor producción de miniestaquillas. La reducción del abono comercial logró incrementar el enraizamiento de las estaquillas y redujo las variaciones en el pH y CE. La producción en larga escala de miniestaquillas de esa espécie traz el miniestaquillado es posible especialmente se utilizando bajas dosis del nitrógeno en el abonado. Términos para indexación: Propagación asexuada, multiplicación clonal, Myrtaceae NITROGEN DOSES ON ADUBATION IN CLONAL MINI GARDEN Of SURINAM ChERRy (Eugenia uniflora)ABSTRACT -The minicutting is an effective and quick method of vegetative propagation, dedicated to spread of forest and fruit trees, providing the formation of homogeneous orchards. In order to determine the adubation management in multiplication of Surinam cherry trees by minicutting in clonal gardens, we tested increased nitrogen (N) doses in fertilizer formulation, in two stages, over a period of 260 days. The solutions adopted in the first stage were: solution A: 10 g L-1 Kristalon® (NPK 4,(5)(6)(7)(8)(9)8), solution B: 10 g L-1 kristalon® + 3.5 g of urea 8) and solution c: 10 g L-1 kristalon® + 7.0 g of urea 8). In the second step we used half of the fertilizer Kristalon® doses (5 g L-1) and the same additions of urea. We evaluated the production and rooting of minicuttings and we monitored the pH and electrical conductivity (EC) of each solution and of the substrate. The experimental design was the completely randomized design, with seven vessels with five stock plants each per treatment. The first collect of minicuttings occurred seven days after we started the treatment. The solutions A and B promoted greater production of mini cuttings. The reduction of commercial fertilizer increased the rooting and reduced the flutuations in pH and EC values. The production in large scale of cuttings of this species by using this technique is possible particularly when low doses of nitrogen in the fertilizer.

RESUMO -Os fungos micorrízicos arbusculares (FMA) promovem maior desenvolvimento de plantas, entretanto, a simbiose depende das espécies de ambos os simbiontes. Objetivou-se avaliar a interação entre porta-enxertos cítricos inoculados com espécies de fungos micorrízicos arbusculares. Testou-se três porta-enxertos citrangeiro 'Fepagro C37 Reck' [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck.], cunquateiro 'Hong-Kong' (Fortunella hindsii Swingle) e 'Trifoliata' (Poncirus trifoliata) inoculados com duas espécies de FMA (Glomus etunicatum W.N. Becker & Gerd. e Acaulospora sp.) e um tratamento não inoculado. Avaliou-se a altura (cm), o diâmetro do caule (mm), a área foliar (cm²/planta), o número de folhas, a massa fresca e seca da raiz e da parte aérea (g), o teor de reservas nos tecidos e a colonização dos FMA nas raízes. As espécies G. etunicatum e Acaulospora sp. colonizaram as raízes dos porta-enxertos, mas somente foram efi cientes para o 'Fepagro C37 Reck'. Poncirus trifoliata apresentou maior vigor em ausência de FMA. Palavras-chave: endomicorrizas, produção de mudas, simbioseABSTRACT -Interaction between rootstocks citrus and arbuscular mycorrhizal fungi. Arbuscular mycorrhizal fungi (AMF) support plant development, however, symbiosis depends on species of both symbionts. This study aimed to evaluate the interaction between rootstock citrus inoculated with species of arbuscular mycorrhizal fungi. Three rootstocks citrange 'Fepagro C37 Reck' [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck.], 'Hong-Kong' kumquat (Fortunella hindsii Swingle) and 'Trifoliata' (Poncirus trifoliate) were tested, inoculated with two species of AMF (Glomus etunicatum W.N. Becker & Gerd.and Acaulospora sp.) and a treatment not inoculated. We evaluated the height (cm), stem diameter (mm), leaf area (cm² / plant), leaf number, fresh and dry root and shoot weight (g), the level of reserves in tissues and the colonization of AMF in the roots. The species G. etunicatum and Acaulospora sp. colonized the roots of rootstocks, but they were only eff ective for 'Fepagro C37 Reck'. Poncirus trifoliata had the highest vigor in the absence of AMF.

The use of arbuscular mycorrhizal fungi (AMF) in the production of rootstocks is a strategy to promote faster plant growth. However, this response depends on the symbiont species. The objective of this study was to evaluate the influence of different species of arbuscular mycorrhizal fungus (AMF) on the development and physiology of citrange 'Fepagro C37 Reck' rootstock. Four species of AMF were used in the experiment: Scutellospora heterogama, Gigaspora margarita, Glomus etunicatum, and Acaulospora sp., and a control treatment. The experiment was arranged in a randomized blocks design, with 5 treatments, 3 replicates, and 10 plants per plot. Every 15 days, height (cm) and stem diameter (mm) of plants were measured in the nursery. At 330 days, the number of leaves, leaf area (cm²), fresh and dry mass of roots and shoot (g/plant), root and shoot reserve content, and AMF colonization were evaluated. The AMF inoculation promoted an accelerated vegetative growth, resulting in greater height and diameter. The fungi also promoted greater absorption of most of the nutrients and increase in all other parameters evaluated. However, no effect was found on the reserve content of the rootstock. Roots were colonized with moderate presence of arbuscules and low presence of vesicles. AMF, regardless of species, influence the development and physiology of the citrange 'Fepagro C37 Reck' rootstock.

A interação entre espécies vegetais e micorrizas arbusculares (MA) favorece o desenvolvimento de mudas. Para verificar esta interação conduziu-se estudo, em duas etapas, entre três espécies de micorrizas arbusculares (Glomus etunicatum, Gigaspora margarita e Scutellospora heterogama), além de uma testemunha e três espécies de Myrtaceae nativas no Sul do Brasil: Eugenia pyriformis Cambess, Myrcianthes pungens (O. Berg) D. Legrand) e Psidium cattleyanum Sabine na produção de mudas. Na etapa de sementeira, sementes das espécies vegetais foram semeadas em bandejas multicelulares, preenchidas com substrato autoclavado e 1 g de inóculo/célula de cada espécie de MA estudada. Nesta etapa avaliaram-se a emergência e o desenvolvimento inicial das plântulas (altura e número de folhas por muda), aos 125 dias após a semeadura. Na etapa de viveiro, as mudas foram transplantadas para sacos de polietileno preto (5 L), contendo substrato comercial desinfestado. 480 dias após o transplante, avaliaram-se o desenvolvimento vegetativo das plantas e a colonização pelas MAs nas raízes. Na fase de sementeira, as MAs colonizaram as raízes, mas não alteraram emergência e desenvolvimento inicial das plântulas. Após o transplante para recipientes maiores todas as espécies de MAs foram eficientes quando comparadas à testemunha, proporcionando incrementos médios na ordem de 135% na altura, 195% no número de folhas, 299% no número de brotações e 209% na área foliar. Os resultados confirmam a efetiva simbiose entre as espécies florestais e as endomicorrizas estudadas.

A produção de mudas de maracujazeiro-azedo em ambiente protegido é uma tecnologia diferenciada, que qualifica o setor produtivo a reduzir ou até eliminar o risco de disseminação de doenças e pragas através das mudas. Também proporciona a obtenção de mudas avançadas (maiores que 80cm de altura), que possibilitam antecipação de colheita e maior produtividade em áreas de ocorrência endêmica da virose do endurecimento dos frutos do maracujazeiro, principal doença dessa cultura. Termos para indexação: Passiflora edulis; virose do endurecimento dos frutos; mudas sadias.

Rootstocks of ‘Rangpur’ Lime and ‘Swingle’ Citrumelo were grown in containers with substrate in a greenhouse, aiming to evaluate the effects of N (urea) fertilization on the vegetative growth and macronutrient content of the plant tissue. The experimental design was a factorial randomized block design with four repetitions, and each experimental plot was composed of five plants. Four doses of N (0, 2.0, 4.0 and 8.0 g.plant-1) were evaluated and applied every week (15 applications) to both of the rootstocks. After 200 days of transplanting, the following parameters were evaluated: vegetative growth and total content of macronutrients on the dry weight of the leaves, stems, and roots. ‘Rangpur’ Lime was more vigorous that ‘Swingle’ Citrumelo. ‘Rangpur’ Lime showed the greatest accumulation of plant dry weight with 3.38 g.plant-1 of N and a greater root dry weight with 2.03 g.plant-1. For ‘Swingle’ Citrumelo, 2.03 g.plant-1 of N provided a greater plant dry weight, however, nitrogen fertilization reduced the root:canopy ratio of the rootstocks. The leaf content of N and P were favored by high doses of N in the tested range. Intermediate doses favored the Ca and Mg leaf contents. The leaf K content was decreased by nitrogen fertilization.

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